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Data in support of Circulating neutrophil extracellular trap components including cell-free DNA are associated with neonatal sepsis and necrotizing enterocolitis in newborn, immature infants, pigs and mice

ABSTRACT: Preterm infants are highly susceptible to late-onset sepsis (LOS) and necrotizing enterocolitis (NEC) but specific biomarkers for diagnosis and effective treatment are lacking. Neutrophil extracellular traps (NETs) are related to sepsis in adults but not investigated in infant conditions. This is the first proteome study to document that circulating NETs are involved in neonatal LOS and NEC. cfDNA and NET proteins may provide new potential diagnostic markers for these diseases.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Blood Plasma

SUBMITTER: Allan Stensballe

LAB HEAD: Allan Stensballe

PROVIDER: PXD004082 | Pride | 2018-03-05

REPOSITORIES: Pride

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Publications

Elevated levels of circulating cell-free DNA and neutrophil proteins are associated with neonatal sepsis and necrotizing enterocolitis in immature mice, pigs and infants.

Nguyen Duc Ninh DN Stensballe Allan A Lai Jacqueline Cy JC Jiang Pingping P Brunse Anders A Li Yanqi Y Sun Jing J Mallard Carina C Skeath Tom T Embleton Nicholas D ND Berrington Janet E JE Sangild Per T PT

Innate immunity 20170717 6

Preterm infants are highly susceptible to late-onset sepsis (LOS) and necrotizing enterocolitis (NEC), but disease pathogenesis and specific diagnostic markers are lacking. Circulating cell-free DNA (cfDNA) and immune cell-derived proteins are involved in multiple immune diseases in adults but have not been investigated in preterm neonates. We explored the relation of circulating neutrophil-associated proteins and cfDNA to LOS and/or NEC. Using a clinically relevant preterm pig model of spontane ...[more]

PMID: 28714327

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