Dataset Information

Qualitative and quantitative analysis of Bothrops jararaca venom gland proteome

ABSTRACT: While cellular transcripts encode rich information that provide key features to understand the molecular basis of snake venom variation, their presence/ abundance does not necessary imply/correlate in the translation of a functional (protein) product. In this study we carried out an analysis of the venom gland proteome of Bothrops jararaca taking into account two distinct phases of its ontogenetic development (i.e. newborn and adult specimens) and the marked sexual dimorphism recently reported on its venom proteome. Proteomic data analysis showed wider dynamic range for toxins when comparing to non-toxins and a dynamic proteome rearrangement in cellular proteins upon B. jararaca development. Differentially expressed proteins covered a number of biological pathways related to protein synthesis, including proteins related to transcription and translation, which were found to be significantly higher expressed in the newborn venom gland. Our results showed that the variation in the expression levels of cellular proteins gives rise to an even higher variation in the dynamic range of the expressed toxins. Upon ageing, the molecular constraints related to protein synthesis together with ecological traits would likely have an impact on the toxin repertoire, which, in the case of B. jararaca species, would enable the species to deal with different prey types during its lifespan.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Bothrops Jararaca (jararaca) (bothrops Jajaraca)

TISSUE(S): Venom Gland

SUBMITTER: Andre Zelanis

LAB HEAD: André Zelanis

PROVIDER: PXD004186 | Pride | 2016-09-28

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	OB_AZP_A_R1_20151117_32.raw	Raw
	OB_AZP_A_R2_20151117_33.raw	Raw
	OB_AZP_A_R3_20151117_34.raw	Raw
	OB_AZP_P_R1_20151117_35.raw	Raw
	OB_AZP_P_R2_20151117_36.raw	Raw

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Publications

Dynamic Rearrangement in Snake Venom Gland Proteome: Insights into Bothrops jararaca Intraspecific Venom Variation.

Augusto-de-Oliveira César C Stuginski Daniel R DR Kitano Eduardo S ES Andrade-Silva Débora D Liberato Tarcísio T Fukushima Isabella I Serrano Solange M T SM Zelanis André A

Journal of proteome research 20160912 10

We carried out an analysis of the venom gland proteome of Bothrops jararaca taking into account two distinct phases of its ontogenetic development (i.e., newborn and adult) and the marked sexual dimorphism recently reported on its venom proteome. Proteomic data analysis showed a dynamic rearrangement in the proteome landscape of B. jararaca venom gland upon development and gender-related changes. Differentially expressed proteins covered a number of biological pathways related to protein synthes ...[more]

PMID: 27575776

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