Dataset Information

Diurnal regulatory landscape of the mouse liver nucleus

ABSTRACT: To dissect how diurnal rhythms affect key functions such as transcription or chromatin remodelling, we quantified the temporal nuclear accumulation of proteins and phosphoproteins from mouse liver using in vivo stable isotope labelling by amino acids in cell culture (SILAC)-based mass spectrometry (MS). Protein extracts from isotope labelled mice liver nuclei were used as a reference and mixed with extracts from animals collected every 3 h for 45 h total. Phosphopeptide levels were analysed after enrichment with titanium dioxide (TiO2). The proteins levels were also analysed (dataset with project accession PXD003818).

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Hepatocyte, Liver

SUBMITTER: Loïc Dayon

LAB HEAD: Frédéric Gachon

PROVIDER: PXD004191 | Pride | 2016-12-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MaxQuant_Output.zip	Other
	PhosphoSTYSites_Nucleus_export.xlsx	Xlsx
	Phospho_nuclei_Sample_1.zip	Other
	Phospho_nuclei_Sample_10.zip	Other
	Phospho_nuclei_Sample_11.zip	Other

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Publications

Nuclear Proteomics Uncovers Diurnal Regulatory Landscapes in Mouse Liver.

Wang Jingkui J Mauvoisin Daniel D Martin Eva E Atger Florian F Galindo Antonio Núñez AN Dayon Loïc L Sizzano Federico F Palini Alessio A Kussmann Martin M Waridel Patrice P Quadroni Manfredo M Dulić Vjekoslav V Naef Felix F Gachon Frédéric F

Cell metabolism 20161103 1

Diurnal oscillations of gene expression controlled by the circadian clock and its connected feeding rhythm enable organisms to coordinate their physiologies with daily environmental cycles. While available techniques yielded crucial insights into regulation at the transcriptional level, much less is known about temporally controlled functions within the nucleus and their regulation at the protein level. Here, we quantified the temporal nuclear accumulation of proteins and phosphoproteins from mo ...[more]

PMID: 27818260

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