Dataset Information

Proteomic characterization of Rathayibacter toxicus FH 79 using LC MS/MS

ABSTRACT: Rathayibacter toxicus is a gram-positive bacterium that is the causative agent of annual ryegrass toxicity, a disease that causes devastating losses in the Australian livestock industry. This bacterium is poorly characterized, making it difficult to accurately detect in feed samples. Using 1-D gels and mass spectrometry, we analyzed the protein expression of R. toxicus under stationary growth phase conditions to obtain a more complete understanding of the mechanisms of this organism. A total of 333 unique proteins were identified. The data obtained in this analysis is an essential first step toward developing an antibody-based diagnostic assay.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Rathayibacter Toxicus

SUBMITTER: Michael McMahon

LAB HEAD: Michael Boyd McMahon

PROVIDER: PXD004238 | Pride | 2018-01-15

REPOSITORIES: Pride

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Publications

Partial Proteome of the Corynetoxin-Producing Gram-Positive Bacterium, Rathayibacter toxicus.

Fennessey Christine M CM McMahon Michael B MB Sechler Aaron J AJ Kaiser Jaclyn J Garrett Wesley M WM Tancos Matthew A MA Luster Douglas G DG Rogers Elizabeth E EE Schneider William L WL

Proteomics 20180202 3-4

Rathayibacter toxicus is a Gram-positive bacterium that is the causative agent of annual ryegrass toxicity (ARGT), a disease that causes devastating losses in the Australian livestock industry. R. toxicus exhibits a complex life cycle, using the nematode Anguina funesta as a physical vector to carry it up to the seed head of the host plant. ARGT is caused by a tunicamycin-like corynetoxin that is produced in R. toxicus-infected seed galls. We analyzed protein expression in R. toxicus under stati ...[more]

PMID: 29327412

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Project description:In response to infection, viral genomes are processed by Dicer-like (DCL) ribonuclease proteins into viral small RNAs (vsRNAs) of discrete sizes. vsRNAs are then used as guides for silencing the viral genome. The profile of vsRNAs produced during the infection process has been extensively studied for some group of viruses. However, nothing is known for members of the economically important family Luteoviridae, a group of phloem-restricted viruses. Here, the population of vsRNAs from cotton plants infected with Cotton leafroll dwarf virus (CLRDV), a member of the genus Polerovirus, family Luteoviridae, is characterized.Deep sequencing of small RNAs (sRNAs) from CLRDV-infected cotton leaves was performed. Results showed 21-nt to 24-nt long vsRNAs matching all the viral genome, with a higher frequency of matches in the 3M-CM-"M-BM-^@M-BM-^Y region. Equivalent amounts of sense and antisense vsRNAs were found, and the 22-nt long small RNA class was the most prominent one. Looking for cotton Dcl transcripts levels during infection, we could observe that Dcl4 seems to be up-regulated, while Dcl2 seems to be down-regulated.This is the first report on the profile of sRNAs coming from a plant infected with a member of the family Luteoviridae. Our sequence data strongly suggest that virus-derived double-stranded RNA functions as one of the main precursors of vsRNAsOur results indicate that secondary structures of the viral RNAs are not the main source of the viRNAs observed, as suggested for other viruses. Judging by the profiled size classes, all cotton DCLs might be working to silence the virus. The possible causes for the unexpected high accumulation of 22-nt viRNAvsRNAs are discussed. CLRDV is the causal agent of worldwide cotton pathology named Cotton blue disease. Our results are an important contribution for understanding the molecular mechanisms involved in this and related diseases. Total RNA obtained from leaves of the cotton plant 5 days post-infection with Cotton leafrol dawrf virus (CLRDV) compared to not-infected control.

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Dataset Information

Proteomic characterization of Rathayibacter toxicus FH 79 using LC MS/MS

Dataset's files

Publications

Partial Proteome of the Corynetoxin-Producing Gram-Positive Bacterium, Rathayibacter toxicus.

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