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Proteomic analysis of the immune response to dsRNA in B.dorsalis

ABSTRACT: Proteomic analysis of a mixed sample from 8, 12 and 24 h (early set) post first exposure to dsRNA and a sample from 5 days (late set) post first exposure to dsRNA to identify differentially expressed proteins which were responsible to the immune response to dsRNA in B.dorsalis.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Bactrocera Dorsalis

TISSUE(S): Whole Body

SUBMITTER: Xiaolong Dong

LAB HEAD: Xiaolong Dong

PROVIDER: PXD004272 | Pride | 2018-10-27

REPOSITORIES: Pride

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			Action	DRS
	Bactrocera_all.dat	Other
	raw1.rar	Other

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Publications

The inducible blockage of RNAi reveals a role for polyunsaturated fatty acids in the regulation of dsRNA-endocytic capacity in Bactrocera dorsalis.

Dong Xiaolong X Li Xiaoxue X Li Qiujia Q Jia Hongmei H Zhang Hongyu H

Scientific reports 20170717 1

Exogenous double-stranded RNA (dsRNA) can trigger gene silencing through the RNA interference (RNAi) pathway. Our previous research established that Bactrocera dorsalis can block RNAi after an initial priming of exposure to dsRNA. However, the mechanism underlying this phenomenon is not yet fully understood. Here, we demonstrate that fatty acid biosynthesis and metabolism pathways play important roles in the blockage of RNAi induced by dsRNA priming. The ratio of linoleic acid (LA) to arachidoni ...[more]

PMID: 28717187

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