Dataset Information

Comparative ploidy proteomics of Candida albicans biofilms unraveled the role of AHP1 in the biofilm persistence against amphotericin B

ABSTRACT: To understand the molecular mechanism operating in the biofilm mode of growth of C. albicans haploids, we performed an iTRAQ-based mass spectrometry profiling of proteins of 72 h mature biofilms of the two haploids, GZY792 and GZY803 compared to that of the diploids, SC5314 and BWP17.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Candida Albicans (yeast)

SUBMITTER: Thuyen Truong

LAB HEAD: Dr. C. J. Seneviratne

PROVIDER: PXD004274 | Pride | 2016-09-27

REPOSITORIES: Pride

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Comparative Ploidy Proteomics of Candida albicans Biofilms Unraveled the Role of the AHP1 Gene in the Biofilm Persistence Against Amphotericin B.

Truong Thuyen T Zeng Guisheng G Qingsong Lin L Kwang Lim Teck LT Tong Cao C Chan Fong Yee FY Wang Yue Y Seneviratne Chaminda Jayampath CJ

Molecular & cellular proteomics : MCP 20160919 11

Candida albicans is a major fungal pathogen causing lethal infections in immunocompromised patients. C. albicans forms antifungal tolerant biofilms contributing significantly to therapeutic failure. The recently established haploid C. albicans biofilm model provides a new toolbox to uncover the mechanism governing the higher antifungal tolerance of biofilms. Here, we comprehensively examined the proteomics and antifungal susceptibility of standard diploid (SC5314 and BWP17) and stable haploid (G ...[more]

PMID: 27644984

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Project description:Background and aimsDespite the recent wealth of studies targeted at contact zones of cytotypes in various species, some aspects of polyploid evolution are still poorly understood. This is especially the case for the frequency and success rate of spontaneous neopolyploidization or the temporal dynamics of ploidy coexistence, requiring massive ploidy screening and repeated observations, respectively. To fill this gap, an extensive study of spatio-temporal patterns of ploidy coexistence was initiated in the widespread annual weed Tripleurospermum inodorum (Asteraceae).MethodsDNA flow cytometry along with confirmatory chromosome counts was employed to assess ploidy levels of 11?018 adult individuals and 1263 ex situ germinated seedlings from 1209 Central European populations. The ploidy screening was conducted across three spatial scales and supplemented with observations of temporal development of 37 mixed-ploidy populations.Key resultsThe contact zone between the diploid and tetraploid cytotypes has a diffuse, mosaic-like structure enabling common cytotype coexistence from the within-population to the landscape level. A marked difference in monoploid genome size between the two cytotypes enabled the easy distinction of neotetraploid mutants from long-established tetraploids. Neotetraploids were extremely rare (0·03?%) and occurred solitarily. Altogether five ploidy levels (2 x -6 x ) and several aneuploids were discovered; the diversity in nuclear DNA content was highest in early ontogenetic stages (seedlings) and among individuals from mixed-ploidy populations. In spite of profound temporal oscillations in cytotype frequencies in mixed-ploidy populations, both diploids and tetraploids usually persisted up to the last census.ConclusionsDiploids and tetraploids commonly coexist at all spatial scales and exhibit considerable temporal stability in local ploidy mixtures. Mixed-ploidy populations containing fertile triploid hybrids probaby act as effective generators of cytogenetic novelty and may facilitate inter-ploidy gene flow. Neopolyploid mutants were incapable of local establishment.