Dataset Information

Quantitative Secretome Analysis of Activated Jurkat Cells

ABSTRACT: A quantitative label-free secretome analysis protocol using a click chemistry-based approach for the enrichment of secreted glycoproteins was adapted for and applied to a T cell model. There, Jurkat cells were activated via PMA/ionomycin and the dynamic modulation of the T cell secretome was investigated and compared to the dynamic modulation of the T cell proteome of the same model.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell, Jurkat Cell

DISEASE(S): T-cell Leukemia

SUBMITTER: Thilo Bracht

LAB HEAD: Barbara Sitek

PROVIDER: PXD004280 | Pride | 2016-10-10

REPOSITORIES: Pride

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Quantitative Secretome Analysis of Activated Jurkat Cells Using Click Chemistry-Based Enrichment of Secreted Glycoproteins.

Witzke Kathrin E KE Rosowski Kristin K Müller Christian C Ahrens Maike M Eisenacher Martin M Megger Dominik A DA Knobloch Jürgen J Koch Andrea A Bracht Thilo T Sitek Barbara B

Journal of proteome research 20161019 1

Quantitative secretome analyses are a high-performance tool for the discovery of physiological and pathophysiological changes in cellular processes. However, serum supplements in cell culture media limit secretome analyses, but serum depletion often leads to cell starvation and consequently biased results. To overcome these limiting factors, we investigated a model of T cell activation (Jurkat cells) and performed an approach for the selective enrichment of secreted proteins from conditioned med ...[more]

PMID: 27696881

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