Dataset Information

Social Network Architecture of Human Immune Cells Unveiled by Quantitative Proteomics

ABSTRACT: The immune system is unique in its dynamic interplay between numerous cell types. However, a system-wide view of how immune cells communicate to protect against disease has not been established. Here, we applied high-resolution mass spectrometry-based proteomics to generate a publicly accessible protein atlas of 28 primary human immune cell populations in steady and activated states at a depth of > 10,000 proteins. Cell-type-specific protein copy numbers reveal that immune cells are most specialized at the level of ligands and receptors, thereby connecting distinct functions of the immune system. By integrating total and secreted proteomes, we deduce paracrine immune dynamics upon microbial encounter and discover fundamental intercellular communication structures as well as novel connections between cellular senders and receivers of biological information. Our comprehensive cell-type-resolved proteomic resource of human immune cells provides a framework for the orchestration of cellular interplay and a reference for altered communication associated with pathology.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood

SUBMITTER: Mario Oroshi

LAB HEAD: Matthias Mann

PROVIDER: PXD004352 | Pride | 2017-03-15

REPOSITORIES: Pride

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Source:

			Action	DRS
	20131115_EXQ2_JaRi_SA_Do132_Memory_Free_Act_01.raw	Raw
	20131115_EXQ2_JaRi_SA_Do132_Memory_Free_Act_02.raw	Raw
	20131115_EXQ2_JaRi_SA_Do132_Memory_Free_Act_03.raw	Raw
	20131115_EXQ2_JaRi_SA_Do132_Memory_Free_CTL_01.raw	Raw
	20131115_EXQ2_JaRi_SA_Do132_Memory_Free_CTL_02.raw	Raw

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Social network architecture of human immune cells unveiled by quantitative proteomics.

Rieckmann Jan C JC Geiger Roger R Hornburg Daniel D Wolf Tobias T Kveler Ksenya K Jarrossay David D Sallusto Federica F Shen-Orr Shai S SS Lanzavecchia Antonio A Mann Matthias M Meissner Felix F

Nature immunology 20170306 5

The immune system is unique in its dynamic interplay between numerous cell types. However, a system-wide view of how immune cells communicate to protect against disease has not yet been established. We applied high-resolution mass-spectrometry-based proteomics to characterize 28 primary human hematopoietic cell populations in steady and activated states at a depth of >10,000 proteins in total. Protein copy numbers revealed a specialization of immune cells for ligand and receptor expression, ther ...[more]

PMID: 28263321

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