Proteomics

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HILIC and ERLIC Enrichment of Glycopeptides Derived from Breast and Brain Cancer Cells

ABSTRACT: Aberrant glycosylation has been linked to many different cancer types. The blood brain barrier (BBB) is a region of the brain that regulates the entrance of ions, diseases, toxins, etc. However in breast cancer metastasis, the BBB fails to prevent the crossing of the cancer cells into the brain. Here we present a study of identifying and quantifying the glycosylation of six breast and brain cancer cell lines using hydrophilic interaction liquid chromatography (HILIC) and electrostatic repulsion liquid chromatography (ERLIC) enrichments and LC-MS/MS analysis. Qualitative and quantitative analyses of N-linked glycosylation were performed by both enrichment techniques for individual and complementary comparison. Potential cancer glycopeptide biomarkers were identified and confirmed by chemometric and statistical evaluations. The results provide the first comprehensive glycopeptide listing for these six cell lines.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Jingfu Zhao  

LAB HEAD: Yehia Mechref

PROVIDER: PXD004361 | Pride | 2019-02-27

REPOSITORIES: Pride

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Action DRS
126_HIERLIC_091614_01.dat Other
126_HIERLIC_091614_01.raw Raw
126_HIERLIC_091614_02.dat Other
126_HIERLIC_091614_02.raw Raw
126_HIERLIC_091614_03.dat Other
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HILIC and ERLIC Enrichment of Glycopeptides Derived from Breast and Brain Cancer Cells.

Zacharias Lauren G LG   Hartmann Alyssa K AK   Song Ehwang E   Zhao Jingfu J   Zhu Rui R   Mirzaei Parvin P   Mechref Yehia Y  

Journal of proteome research 20160912 10

Aberrant glycosylation has been linked to many different cancer types. The blood-brain barrier (BBB) is a region of the brain that regulates the entrance of ions, diseases, toxins, and so on. However, in breast cancer metastasis, the BBB fails to prevent the crossing of the cancer cells into the brain. Here we present a study of identifying and quantifying the glycosylation of six breast and brain cancer cell lines using hydrophilic interaction liquid chromatography (HILIC) and electrostatic rep  ...[more]

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