Proteomics

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Comparative proteomic analysis of exoproteome and membrane enriched fractions of PAK and PAK pJN2133 strains

ABSTRACT: This work characterizes a P.aeruginosa strain with low levels of c-di-GMP resultant from the overexpression of the phosphodiesterase PA2133. Phenotypic characterization showed that biofilm formation, adhesion to epitheliel cells, and motility are severely impaired in this strain. We applied both, DIGE / MALDI-TOF/TOF and shotgun LC/MS/MS quantitative proteomic analysis to exoproteome and enriched membrane protein fractions. Results from both complementary strategies were in agreement and revealed motility, type III secretion, and chemotaxis to be the main affected processes by PA2133 overexpression. These results are the first to show that flagella synthesis, twitching motility, and chemotaxis are negatively regulated by low c-di-GMP levels in P.aeruginosa and thus confront our current undertsandings of c-di-GMP signalling.

INSTRUMENT(S): LTQ Velos

ORGANISM(S): Pseudomonas Aeruginosa Pak

SUBMITTER: Jessica Rossello  

LAB HEAD: Rosario Durán

PROVIDER: PXD004365 | Pride | 2017-09-05

REPOSITORIES: pride

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PAKexoproteomereplicate1.raw Raw
PAKexoproteomereplicate1.txt Txt
PAKexoproteomereplicate2.raw Raw
PAKexoproteomereplicate2.txt Txt
PAKexoproteomereplicate3.raw Raw
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Publications

The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism.

Rossello Jessica J   Lima Analía A   Gil Magdalena M   Rodríguez Duarte Jorge J   Correa Agustín A   Carvalho Paulo C PC   Kierbel Arlinet A   Durán Rosario R  

Scientific reports 20170831 1

The second messenger c-di-GMP regulates the switch between motile and sessile bacterial lifestyles. A general feature of c-di-GMP metabolism is the presence of a surprisingly large number of genes coding for diguanylate cyclases and phosphodiesterases, the enzymes responsible for its synthesis and degradation respectively. However, the physiological relevance of this apparent redundancy is not clear, emphasizing the need for investigating the functions of each of these enzymes. Here we focused o  ...[more]

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