Dataset Information

Secretome profiling of human airway epithelial cultures infected with respiratory syncytial virus

ABSTRACT: The respiratory epithelium is a polarised layer at the interface between the outside environment and deeper lung structures, overlaid by the epithelial lining fluid (ELF). This provides a mechanical and immunological barrier to inhaled particulates, such as viruses. Human respiratory syncytial virus (hRSV) is a major cause of disease in humans, and targets the respiratory epithelium. However, little is known of the disruption of the ELF proteome in the context of virus-driven respiratory illnesses. To address this, a proteomics approach was combined with an ex-vivo human airway epithelial model (HAE) to investigate the apical and basolateral secretome in hRSV-infected cultures. This demonstrated that several apically- and basolaterally-restricted proteins were subsequently secreted in both directions upon infection, while a number of proteins saw their apical/basolateral abundance ratios significantly altered. Furthermore, another 35 proteins were uniquely identified after hRSV treatment. Importantly, some of these changes were correlated in nasal aspirates (NA) from children with and without hRSV. This study showed that hRSV could affect airway secretions, and disrupted the directionality of the respiratory epithelium.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Respiratory Syncytial Virus Infectious Disease

SUBMITTER: Stuart Armstrong

LAB HEAD: Julian Hiscox

PROVIDER: PXD004430 | Pride | 2022-03-01

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	MQsearchtxt.zip	Other
	SA230714_olivier_secretome_1.raw	Raw
	SA230714_olivier_secretome_10.raw	Raw
	SA230714_olivier_secretome_11.raw	Raw
	SA230714_olivier_secretome_12.raw	Raw

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Publications

The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins.

Touzelet Olivier O Broadbent Lindsay L Armstrong Stuart D SD Aljabr Waleed W Cloutman-Green Elaine E Power Ultan F UF Hiscox Julian A JA

Molecular & cellular proteomics : MCP 20200219 5

The respiratory epithelium comprises polarized cells at the interface between the environment and airway tissues. Polarized apical and basolateral protein secretions are a feature of airway epithelium homeostasis. Human respiratory syncytial virus (hRSV) is a major human pathogen that primarily targets the respiratory epithelium. However, the consequences of hRSV infection on epithelium secretome polarity and content remain poorly understood. To investigate the hRSV-associated apical and basolat ...[more]

PMID: 32075873

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