Dataset Information

Alterations in kernel proteome after infection with Fusarium culmorum in two triticale cultivars with contrasting resistance to Fusarium head blight

ABSTRACT: Triticale was used as a model to recognize new components of molecular mechanism of resistance to Fusarium head blight (FHB) in cereals. Fusarium-damaged kernels of two lines distinct in levels of resistance to FHB were applied into a proteome profiling using two-dimensional gel electrophoresis (2-DE) to create protein maps and mass spectrometry to identify the proteins differentially accumulated between the analyzed lines. The 2-DE analysis indicated a total of 23 spots with clear differences in a protein content between the more resistant and more susceptible triticale lines after infection with F. culmorum. A majority of the proteins were involved in a cell carbohydrate metabolism, stressing the importance of this protein group in a plant response to Fusarium infection. The increased accumulation levels of different isoforms of plant beta-amylase were observed for a more susceptible triticale line after inoculation. The more resistant line was characterized by a higher abundance of alpha-amylase inhibitor CM2 subunit.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Secale Cereale X Triticum Turgidum Subsp. Durum

TISSUE(S): Leaf

SUBMITTER: Agata Malinowska

LAB HEAD: Michal Dadlez

PROVIDER: PXD004464 | Pride | 2018-10-27

REPOSITORIES: Pride

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Alterations in Kernel Proteome after Infection with Fusarium culmorum in Two Triticale Cultivars with Contrasting Resistance to Fusarium Head Blight.

Perlikowski Dawid D Wiśniewska Halina H Kaczmarek Joanna J Góral Tomasz T Ochodzki Piotr P Kwiatek Michał M Majka Maciej M Augustyniak Adam A Kosmala Arkadiusz A

Frontiers in plant science 20160817

Highlight: The level of pathogen alpha-amylase and plant beta-amylase activities could be components of plant-pathogen interaction associated with the resistance of triticale to Fusarium head blight. Triticale was used here as a model to recognize new components of molecular mechanism of resistance to Fusarium head blight (FHB) in cereals. Fusarium-damaged kernels (FDK) of two lines distinct in levels of resistance to FHB were applied into a proteome profiling using two-dimensional gel electroph ...[more]

PMID: 27582751

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