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Phagosome proteome of activated murine macrophage

ABSTRACT: Proteomics of carboxylated polystyrene bead (1.0 um) phagosomes from murine bone marrow-derived macrophages. cells were either resting or treated with 100 U/ml IFN-γ (PeproTech) and 100 ng/ml LPS (Sigma) for 24 h, 20 ng/ml Interleukin-4 (IL4) (BD Pharmingen) for 48 h, 20 ng/ml Interleukin-13 (IL13), 10 ng/ml Interleukin-10 (IL10)for 48 h or Reprogrammed (IL4 was incubated with BMDMs for 24 h, and the medium was replaced with fresh medium containing IFN-γ/LPS to incubate for another 24 h). Phagosomes were isolated after 30 min bead inoculation.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Macrophage

SUBMITTER: Matthias Trost

LAB HEAD: Matthias Trost

PROVIDER: PXD004520 | Pride | 2019-03-22

REPOSITORIES: Pride

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			Action	DRS
	MaxQuantOutput.zip	Other
	Phagosomes_IFNgamma.raw	Raw
	Phagosomes_IFNgammavsRest_1.raw	Raw
	Phagosomes_IFNgammavsRest_2.raw	Raw
	Phagosomes_IFNgammavsRest_3.raw	Raw

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