Proteomics

Dataset Information

0

High-throughput Proteomics Reveals the Unicellular Roots of Animal Phosphosignaling and Cell Differentiation


ABSTRACT: Cell-specific regulation of protein levels and activity is essential for the distribution of functions among multiple cell types in animals. The finding that many genes involved in these regulatory processes have a premetazoan origin raises the intriguing possibility that the mechanisms required for spatially regulated cell differentiation evolved prior to the appearance of animals. Here, we use high-throughput proteomics in Capsaspora owczarzaki, a close unicellular relative of animals, to characterize the dynamic proteome and phosphoproteome profiles of three temporally distinct cell types in this premetazoan species. We show that life-cycle transitions are linked to extensive proteome and phosphoproteome remodeling and that they affect key genes involved in animal multicellularity, such as transcription factors and tyrosine kinases. The observation of shared features between Capsaspora and metazoans indicates that elaborate and conserved phosphosignaling and proteome regulation supported temporal cell-type differentiation in the unicellular ancestor of animals.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Capsaspora Owczarzaki

SUBMITTER: Eduard Sabidó  

LAB HEAD: Eduard Sabidó

PROVIDER: PXD004567 | Pride | 2016-11-30

REPOSITORIES: Pride

altmetric image

Publications

High-Throughput Proteomics Reveals the Unicellular Roots of Animal Phosphosignaling and Cell Differentiation.

Sebé-Pedrós Arnau A   Peña Marcia Ivonne MI   Capella-Gutiérrez Salvador S   Antó Meritxell M   Gabaldón Toni T   Ruiz-Trillo Iñaki I   Sabidó Eduard E  

Developmental cell 20161013 2


Cell-specific regulation of protein levels and activity is essential for the distribution of functions among multiple cell types in animals. The finding that many genes involved in these regulatory processes have a premetazoan origin raises the intriguing possibility that the mechanisms required for spatially regulated cell differentiation evolved prior to the appearance of animals. Here, we use high-throughput proteomics in Capsaspora owczarzaki, a close unicellular relative of animals, to char  ...[more]

Similar Datasets

2024-08-19 | PXD048644 | Pride
2016-05-25 | PXD001812 | Pride
2017-03-20 | PXD005209 | Pride
2015-08-04 | E-GEOD-65356 | biostudies-arrayexpress
2023-05-09 | PXD036951 | Pride
2012-11-29 | E-GEOD-40155 | biostudies-arrayexpress
2021-05-25 | PXD020087 | Pride
2012-09-06 | E-GEOD-35097 | biostudies-arrayexpress
2015-03-31 | E-GEOD-56325 | biostudies-arrayexpress
2015-03-31 | E-GEOD-56324 | biostudies-arrayexpress