Proteomics

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Integration of two in-depth proteomics approaches determines the high grade serous ovarian cancer-associated kallikrein-related peptidase 7 (KLK7) degradome

ABSTRACT: Kallikrein-related peptidase 7 (KLK7) is a serine peptidase that is over-produced in the most lethal form of ovarian cancer, called high grade serous ovarian cancer (HGSOC). To determine its underlying molecular mechanism of action, a comprehensive analysis of KLK7 degradome in the OVMZ-6 cell-secreted proteins was performed using two complementary proteomic approaches, quantitative PROtein Topography and Migration Analysis Platform (qPROTOMAP) and Terminal Amine Isotopic Labelling of Substrates (TAILS).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Lakmali Munasinghage Silva  

LAB HEAD: D. Prof Judith Ann Clements

PROVIDER: PXD004591 | Pride | 2019-02-15

REPOSITORIES: Pride

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Dataset's files

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Action DRS
OK20141102_MLS_TAILS_OV1.raw Raw
OK20141102_MLS_TAILS_OV1.tandem-pride.pride.mgf.gz Mgf
OK20141102_MLS_TAILS_OV1.tandem-pride.pride.mztab.gz Mztab
OK20141102_MLS_TAILS_OV1.tandem-pride.xml.gz Xml
OK20141102_MLS_TAILS_OV2.raw Raw
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Publications

Integration of Two In-depth Quantitative Proteomics Approaches Determines the Kallikrein-related Peptidase 7 (KLK7) Degradome in Ovarian Cancer Cell Secretome.

Silva Lakmali Munasinghage LM   Kryza Thomas T   Stoll Thomas T   Hoogland Christine C   Dong Ying Y   Stephens Carson Ryan CR   Hastie Marcus Lachlan ML   Magdolen Viktor V   Kleifeld Oded O   Gorman Jeffrey John JJ   Clements Judith Ann JA  

Molecular & cellular proteomics : MCP 20190131 5

Kallikrein-related peptidase 7 (KLK7) is a serine peptidase that is over expressed in ovarian cancer. <i>In vitro</i> functional analyses have suggested KLK7 to play a cancer progressive role, although monitoring of KLK7 expression has suggested a contradictory protective role for KLK7 in ovarian cancer patients. In order to help delineate its mechanism of action and thereby the functional roles, information on its substrate repertoire is crucial. Therefore, in this study a quantitative proteomi  ...[more]

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