Proteomics

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Repo-Man/PP1 regulates heterochromatin formation in interphase

ABSTRACT: GST:Repo-Man(C-terminus) or GST:alone were expressed as in Vagnarelli et al. 2011. Before elution, beads were first incubated with nucleosomes extracted from HeLa. HeLa cells were lysed and nucleosomes were digested with MNase (20min, 37C). Nucleosomes were firstly incubated with GST alone for 2hrs. This cleared lysate was then incubated with GST:Repo-Man and GST alone. Beads were then eluted and mix with 3x Laemli Buffer. Histone bands were sent to Mass Spec.

OTHER RELATED OMICS DATASETS IN: PRJNA327850

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Paola Vagnarelli  

LAB HEAD: Paola Vagnarelli

PROVIDER: PXD004613 | Pride | 2016-12-07

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
E150416_02_Quty1_CS_CO_220_Ines_CtermGST_1.raw Raw
E150416_03_Quty1_CS_CO_220_Ines_CtermGST_2.raw Raw
E150416_04_Quty1_CS_CO_220_Ines_CtermGST_3.raw Raw
E150416_06_Quty1_CS_CO_220_Ines_GSTalone_1.raw Raw
E150416_07_Quty1_CS_CO_220_Ines_GSTalone_2.raw Raw
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Publications

Repo-Man is a protein phosphatase 1 (PP1) targeting subunit that regulates mitotic progression and chromatin remodelling. After mitosis, Repo-Man/PP1 remains associated with chromatin but its function in interphase is not known. Here we show that Repo-Man, via Nup153, is enriched on condensed chromatin at the nuclear periphery and at the edge of the nucleopore basket. Repo-Man/PP1 regulates the formation of heterochromatin, dephosphorylates H3S28 and it is necessary and sufficient for heterochro  ...[more]

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