Dataset Information

Identification of novel Coronin 1A interacting proteins

ABSTRACT: Severe combined immunodeficiency 8 (IMD8) is caused by mutations in the human Coronin 1A (Coro1A). The clinical presentation of IMD8 patients is characterized by recurrent bacterial infections, suggesting an important role of Coro1A in innate immunity. To analyze the molecular mechanism of Coro1A during neutrophil recruitment, we identified the Coro1A interactome by conducting co-immunoprecipitation (Co-IP) experiments using GFP NanoTrap technology and subsequent mass spectrometry (LC-MS/MS) using human neutrophil-like differentiated HL-60 (dHL-60) cells stably expressing Coro1A-EGFP (dHL-60-Coro1A-EGFP) cells.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Leukocyte

SUBMITTER: Ignasi Forne

LAB HEAD: Barbara Walzog

PROVIDER: PXD004635 | Pride | 2017-06-29

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MaxQuant_Output.zip	Other
	Ref538_191115_sample01.raw	Raw
	Ref538_191115_sample02.raw	Raw
	Ref538_191115_sample03.raw	Raw
	Ref538_191115_sample04.raw	Raw

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Publications

Coronin 1A, a novel player in integrin biology, controls neutrophil trafficking in innate immunity.

Pick Robert R Begandt Daniela D Stocker Thomas J TJ Salvermoser Melanie M Thome Sarah S Böttcher Ralph T RT Montanez Eloi E Harrison Ute U Forné Ignasi I Khandoga Alexander G AG Coletti Raffaele R Weckbach Ludwig T LT Brechtefeld Doris D Haas Rainer R Imhof Axel A Massberg Steffen S Sperandio Markus M Walzog Barbara B

Blood 20170614 7

Trafficking of polymorphonuclear neutrophils (PMNs) during inflammation critically depends on the β<sub>2</sub> integrins lymphocyte function-associated antigen 1 (LFA-1) (CD11a/CD18) and macrophage-1 antigen (CD11b/CD18). Here, we identify coronin 1A (Coro1A) as a novel regulator of β<sub>2</sub> integrins that interacts with the cytoplasmic tail of CD18 and is crucial for induction of PMN adhesion and postadhesion events, including adhesion strengthening, spreading, and migration under flow co ...[more]

PMID: 28615221

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