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A phosphoproteomic analysis of ABA induced protein phosphorylation revealed that Serine 71 is a major phosphosite for bZIP72 in SAPK10-mediated ABA signaling

ABSTRACT: we focused on the profiling of rice young seedling phosphosites, phosphopeptides and phosphorylation intensity dynamics in response to ABA by employing a label-free, MS-based phosphoproteomic approach

INSTRUMENT(S): Q Exactive

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Leaf

SUBMITTER: Jian Zhang

LAB HEAD: zhangjian

PROVIDER: PXD004705 | Pride | 2018-10-27

REPOSITORIES: Pride

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			Action	DRS
	Rice_phos_BR_12h_20per_F1_R1.raw	Raw
	Rice_phos_BR_12h_20per_F1_R1_peptide.xlsx	Xlsx
	Rice_phos_BR_12h_20per_F1_R1_protein.xlsx	Xlsx
	Rice_phos_BR_12h_20per_F1_R1_protein_peptide.xlsx	Xlsx
	Rice_phos_BR_12h_20per_F1_R2.raw	Raw

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Publications

A Quantitative Proteomic Analysis of Brassinosteroid-induced Protein Phosphorylation in Rice (<i>Oryza sativa</i> L.).

Hou Yuxuan Y Qiu Jiehua J Wang Yifeng Y Li Zhiyong Z Zhao Juan J Tong Xiaohong X Lin Haiyan H Zhang Jian J

Frontiers in plant science 20170407

The group of polyhydroxysteroid phytohormones referred to as the brassinosteroids (BRs) is known to act on plant development and the stress response. BR signal transduction relies largely on protein phosphorylation. By employing a label-free, MS (Mass Spectrometry)-based phosphoproteomic approach, we report here the largest profiling of 4,034 phosphosites on 1,900 phosphoproteins from rice young seedlings and their dynamic response to BR. 1,821 proteins, including kinases, transcription factors ...[more]

PMID: 28439285

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A phosphoproteomic landscape of rice (Oryza sativa L.) tissues

Project description:Here, we first reported the construction of a phosphoproteomic landscape of 6 tissues, including callus, leaves, roots, shoot meristem (SM), young panicles (YP) and mature panicles (MP), from Nipponbare (Oryza sativa ssp. japonica). By employing a non-gel, quantitative phosphoproteomic approach, a total of 4792 phosphopeptides from 2657 phosphoproteins were identified, which were found to be differentially phosphorylated among tissues.

2018-04-11 | PXD005241 | Pride

A comprehensive proteomic survey of ABA-induced protein phosphorylation in rice (Oryza sativa L.)

Project description:In this study, we focused on the profiling of rice young seedling phosphosites, phosphopeptides and phosphorylation intensity dynamics in response to ABA by employing a label-free, MS-based phosphoproteomic approach, and aimed to reveal the biological significance of protein phosphorylation in ABA signaling from the phosphoproteomic data.

2017-01-17 | PXD004939 | Pride

Reciprocal regulation of the TOR kinase and ABA receptor balances plant growth and stress response

Project description:We found that TOR kinase regulates the plant environmental stress responses through phosphorylation at a conserved serine residue of Abscisic acid receptor PYR1/PYLs/RCARs. The phosphorylation inhibits the function of PYLs and prevents the binding of PYLs to abscisic acid and its downstream substrates of PP2C, which blocks the ABA and stress signaling. Our results suggest that plant utilizes this mechanism as a hub to regulate the ABA-mediate stress responses and growth recovery

2017-12-01 | PXD003746 | Pride

mRNA-seq of ros1-3 and ros1-4 with ABA treatment

Project description:To survey transcriptome changes by the mutations of a DNA demethylase ROS1 responding to a phytohormone abscisic acid, we performed the Next-gen sequencing (NGS) associated RNA-seq analysis. Two ROS1 knockout lines (ros1-3, ros1-4; Penterman et al. 2007 [PMID: 17409185]) with the wild-type Col line (wt) were subjected. Three samples (ros1-3, ros1-4 and wt), biological triplicates, ABA or mock treatment, using Illumina HiSeq 2500 system

2016-01-15 | E-GEOD-65016 | biostudies-arrayexpress

Functional analysis of Arabidopsis LSH8 protein involved in ABA signaling pathway

Project description:Functional analysis of Arabidopsis LSH8 protein involved in ABA signaling pathway

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Transcriptional profiling by array of Arabidopsis ABA auxotroph to identify early downstream targets of transcription in response to a small dose of abscisic acid (ABA)

Project description:Abscisic acid (ABA) is a plant hormone that is important in responding to various environmental stresses. Using an ABA auxotroph in Arabidopsis (aba2-2) as the plant material, we would like to identify early downstream targets of transcription in response to a small dose of ABA, 1 uM. We also added cycloheximide to preferentially obtain immediate targets of ABA addition. We believe that using a sensitized background of an ABA auxotroph would yield a set of genes that are very closely regulated by ABA at the transcriptional level. This data set will be used for network analysis of ABA signaling.

2011-10-30 | E-MEXP-3404 | biostudies-arrayexpress

Expression comparasion of rice seedlings of Osabl1 mutant and WT under ABA treatment

Project description:Expression profiles of seedling roots of rice mutant Osabl1 and wild-type under ABA treatment Mutant and wild-type expression profiling were compared and each has two biological repeats.

2011-10-12 | E-GEOD-18529 | biostudies-arrayexpress

Transcriptional profiling of DEX-inducible SNRK3.15 seedlings in the presence of ABA

Project description:Transcriptional profiling of a DEX-inducible SNRK3.15 seedlings in the presence of ABA.

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Expression data of T87 Arabidopsis cultured cells in the presence of ABA, SA, JA, ABA+SA, or ABA+JA for 3hr or 24hr

Project description:Plant hormones involved in environmental stresses, namely abscisic acid (ABA), salicylic acid (SA), and jasmonic acid (JA), have been shown to interact with each other in a complex manner. To address the network of the hormone interactions, we have investigated the changes in expression under multiple hormone treatments, ABA+SA and ABA+JA. We chose cultured cells to remove the difference in the response to hormones among developmental cells or tissues. The cells were treated for 3hr and 24hr to see the rapid or transient response and steady-state response. The obtained data indicate that ABA and SA affect antagonistically, but these hormones affected many genes collaboratively. Indeed, according to the microarray data, there are many genes that responded only to ABA+SA. In addition, the ABA+SA responsive genes also responded to ABA+JA. These data suggest that hormone crosstalk is more complicated than expected and that more systematic analysis is required to untangle the hormone crosstalk network. To investigate the hormonal interactions, Arabidopsis T87 cultured cells were exposed to ABA, SA, or JA alone, or two hormones simultaneously, ABA+SA or ABA+JA, for 3hr and 24 hr. Comparing the data among those treatments, the relationships among these hormones were deduced.

2011-12-01 | E-GEOD-28600 | biostudies-arrayexpress

Abscisic acid (ABA) application to Populus × canescens in hydroponics

Project description:4 weeks old rooted plantlets of P. × canescens (Clone INRA717 1-B4) were cultivated in hydroponics in 2 l pots in Long Ashton nutrient solution in a culture room for 8 weeks before treatments started. Three treatments were applied to the plants: control treatment (-ABA), continuous 100 µM ABA treatment (+ABA) and discontinuous 100 µM ABA treatment (±ABA). ABA was fed to +ABA plants during the whole treatment period of 30 days. ABA was fed to ±ABA plants for three days in two weeks. Developing xylem and mature xylem were collected separately during the harvest and shortly frozen in liquid nitrogen. RNA was extracted from these samples and followed by RNA-sequencing.

2019-01-20 | E-MTAB-7587 | biostudies-arrayexpress