Dataset Information

Chlorella sp. FC2 IITG temporal nitrogen-starvation LC-MSMS

ABSTRACT: Proteomics study in non-model alga Chlorella FC2 IITG (FC2) challenged with nitrogen starvation for 120 h. Three different nitrogen starvation time-points 40 h, 88 h and 120 h was compared with 0 h (nitrogen sufficient condition). Comparative proteomics analysis was performed using 4-plex isobaric tags for relative and absolute quantification (iTRAQ) labelling strategy, followed by chromatographic separation and final generation of tandem mass spectrometry (MS/MS) spectra of tryptic digested peptides on Q-TOF mass spectrometer.

INSTRUMENT(S): Agilent instrument model

ORGANISM(S): Chlorella Sp. Fc2 Iitg

TISSUE(S): Photosynthetic Cell

SUBMITTER: Vineeta Rai

LAB HEAD: Sanjeeva Srivastava

PROVIDER: PXD004712 | Pride | 2018-10-26

REPOSITORIES: Pride

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Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae.

Rai Vineeta V Muthuraj Muthusivaramapandian M Gandhi Mayuri N MN Das Debasish D Srivastava Sanjeeva S

Scientific reports 20170405

To understand the post-transcriptional molecular mechanisms attributing to oleaginousness in microalgae challenged with nitrogen starvation (N-starvation), the longitudinal proteome dynamics of Chlorella sp. FC2 IITG was investigated using multipronged quantitative proteomics and multiple reaction monitoring assays. Physiological data suggested a remarkably enhanced lipid accumulation with concomitant reduction in carbon flux towards carbohydrate, protein and chlorophyll biosynthesis. The proteo ...[more]

PMID: 28378827

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