Dataset Information

Proteome analysis of Arabidopsis thaliana circadian clock mutants

ABSTRACT: The circadian clock is comprised of proteins that form negative feedback loops, which regulate the timing of global gene expression in a coordinated 24 hour cycle. As a result, the plant circadian clock is responsible for regulating numerous physiological processes central to growth and survival. To date, most plant circadian clock studies have relied on diurnal transcriptome changes to elucidate molecular connections between the circadian clock and observable phenotypes in wild-type plants. Here, we have combined high-throughput RNA-sequencing and mass spectrometry to comparatively characterize the lhycca1, prr7prr9, gi and toc1 circadian clock mutant rosette transcriptome and proteome at the end-of-day and end-of-night.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Photosynthetic Cell, Rosette

SUBMITTER: Alexander Graf

LAB HEAD: Alexander Graf

PROVIDER: PXD004763 | Pride | 2018-01-02

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20121015_TiMet_Golm_ClockMutants_Fract2_S1.raw	Raw
	20121015_TiMet_Golm_ClockMutants_Fract2_S10.raw	Raw
	20121015_TiMet_Golm_ClockMutants_Fract2_S11.raw	Raw
	20121015_TiMet_Golm_ClockMutants_Fract2_S12.raw	Raw
	20121015_TiMet_Golm_ClockMutants_Fract2_S13.raw	Raw

Items per page:

1 - 5 of 293

Publications

Parallel analysis of Arabidopsis circadian clock mutants reveals different scales of transcriptome and proteome regulation.

Graf Alexander A Coman Diana D Uhrig R Glen RG Walsh Sean S Flis Anna A Stitt Mark M Gruissem Wilhelm W

Open biology 20170301 3

The circadian clock regulates physiological processes central to growth and survival. To date, most plant circadian clock studies have relied on diurnal transcriptome changes to elucidate molecular connections between the circadian clock and observable phenotypes in wild-type plants. Here, we have integrated RNA-sequencing and protein mass spectrometry data to comparatively analyse the lhycca1, prr7prr9, gi and toc1 circadian clock mutant rosette at the end of day and ...[more]

PMID: 28250106

Similar Datasets

Project description:The spatial organization of genes in the interphase nucleus plays an important role in establishment and regulation of gene expression. The circadian expressed and clock controlled genes represent about 10% of the transcripts in the mammalian cells, however the role of genome topology in the expression of genes in a circadian manner remains to be elucidated. In this study we investigated the characteristics and dynamics of the genomic loci that contact the clock controlled gene Dbp during the circadian cycle. To do so, we combined genome-wide interaction profiling by chromosome conformation capture-on-chip (4C) technology with analyses of circadian gene expression in synchronized mouse embryonic fibroblasts (MEFs). This approach allowed us to elucidate the three-dimensional organization of the genome during the circadian cycle around the clock controlled gene Dbp, paralleling its circadian expression. We found that the Dbp genomic environment remains largely similar during the circadian cycle. However, specific DNA regions change their frequency of interaction with Dbp as the circadian cycle progresses, delineating a Dbp circadian interactome. These specific changes are not present in Bmal1-/- MEF, suggesting that the clock machinery is implicated in shaping the genomic landscape around the Dbp locus. The Dbp interactome is enriched in DNA elements related to the clock system (E boxes). We found a spatial clustering of functionally related genes. The Dbp circadian interactome contains a subset of circadian genes whose expression which closely parallel that for Dbp. Taken together, our results indicate that the Dbp subnuclear environment is organized to privilege the clock directed gene expression program. Wild type mouse embryonic fibroblasts at the circadian times (CT) 22, 26, 30, 34 and 46. Bmal1-/- mouse embrionic fibroblasts at CT 22 and CT 34 Biological replicate using an independent line of wild type mouse embrionic fibroblast at CT 22 and CT 34 This submission represents the chromosome conformation capture-on-chip component of the overall study

Dataset Information

Proteome analysis of Arabidopsis thaliana circadian clock mutants

Dataset's files

Publications

Parallel analysis of <i>Arabidopsis</i> circadian clock mutants reveals different scales of transcriptome and proteome regulation.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets