Dataset Information

Multiplexed temporal quantification of the human plasma peptidome

ABSTRACT: The plasma peptidome contains the entire complement of low molecular weight endogenous peptides derived from secretion, protease activity and post-translational modifications and is a rich source of biomarkers and novel bioactives. To examine the utility of peptidomics we have examined the effects of exercise on the peptidome because exercise is thought to convey many of its physiological benefits via the blood stream. Deep peptidomic analysis was achieved using 2D LC-MS/MS with multiple fragmentation methods and included identification of post-translational modifications and multiplexed temporal quantification. We show the plasma peptidome is subject to transient changes during exercise on a time-scale of minutes that is rapidly reversible during recovery. Known peptide hormones were acutely regulated including insulin, glucagon, ghrelin, bradykinin, cholecystokinin, secretogranins and many others. Acute regulation of proteases and post-translational modifications including glycosylation were observed. These data reveal the power of peptidomic quantification to temporally quantify bioactive peptides, biomarkers and proteolytic activity during pathophysiological processes.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Benjamin Parker

LAB HEAD: Benjamin Parker

PROVIDER: PXD004781 | Pride | 2017-11-21

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	160127_Fusion_BP_ExercisePeptidome_TMT1_1.raw	Raw
	160127_Fusion_BP_ExercisePeptidome_TMT1_10.raw	Raw
	160127_Fusion_BP_ExercisePeptidome_TMT1_11.raw	Raw
	160127_Fusion_BP_ExercisePeptidome_TMT1_12.raw	Raw
	160127_Fusion_BP_ExercisePeptidome_TMT1_13.raw	Raw

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Publications

Multiplexed Temporal Quantification of the Exercise-regulated Plasma Peptidome.

Parker Benjamin L BL Burchfield James G JG Clayton Daniel D Geddes Thomas A TA Payne Richard J RJ Kiens Bente B Wojtaszewski Jørgen F P JFP Richter Erik A EA James David E DE

Molecular & cellular proteomics : MCP 20171005 12

Exercise is extremely beneficial to whole body health reducing the risk of a number of chronic human diseases. Some of these physiological benefits appear to be mediated via the secretion of peptide/protein hormones into the blood stream. The plasma peptidome contains the entire complement of low molecular weight endogenous peptides derived from secretion, protease activity and PTMs, and is a rich source of hormones. In the current study we have quantified the effects of intense exercise on the ...[more]

PMID: 28982716

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