Dataset Information

Altered signaling associated with chronic arsenic exposure in human skin keratinocytes

ABSTRACT: Modulation of signaling pathways upon chronic arsenic exposure remains poorly studied. Here we carried out SILAC-based quantitative phosphoproteomics analysis to dissect the signaling induced upon chronic arsenic exposure in human skin keratinocyte cell line, HaCaT. We identified 4,171 unique phosphosites derived from 2,000 proteins. We observed differential phosphorylation of 406 phosphosites (2-fold) corresponding to 305 proteins. Several pathways involved in cytoskeleton maintenance and organization were found to be significantly enriched (p<0.05). Our data revealed altered phosphorylation of proteins associated with adherens junction remodeling and actin polymerization. Kinases such as protein kinase C iota type (PRKCI), mitogen-activated protein kinase kinase kinase 1 (MAP3K1), tyrosine-protein kinase BAZ1B (BAZ1B) and STE20 like kinase (SLK) were found to be hyperphosphorylated. Our study provides novel insights into signaling perturbations associated with chronic arsenic exposure in human skin keratinocytes.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Skin

SUBMITTER: Harsha Gowda

LAB HEAD: Harsha Gowda

PROVIDER: PXD004868 | Pride | 2017-08-11

REPOSITORIES: Pride

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Altered signaling associated with chronic arsenic exposure in human skin keratinocytes.

Mir Sartaj Ahmad SA Renuse Santosh S Sathe Gajanan G Khan Aafaque Ahmad AA Patil Arun H AH Nanjappa Vishalakshi V Bhat Firdous Ahmad FA Prasad T S Keshava TSK Giri Ashok K AK Chatterjee Aditi A Gowda Harsha H

Proteomics. Clinical applications 20170815 11-12

Modulation of signaling pathways upon chronic arsenic exposure remains poorly studied. Here, we carried out SILAC-based quantitative phosphoproteomics analysis to dissect the signaling induced upon chronic arsenic exposure in human skin keratinocyte cell line, HaCaT. We identified 4171 unique phosphosites derived from 2000 proteins. We observed differential phosphorylation of 406 phosphosites (twofold) corresponding to 305 proteins. Several pathways involved in cytoskeleton maintenance and organ ...[more]

PMID: 28731282

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			Action	DRS
	HaCaT_pST1.pdResult	Other
	HaCaT_pST1.pep.pep.xml	Pepxml
	HaCaT_pST1_.msf	Msf
	HaCaT_pST2.msf	Msf
	HaCaT_pST2.pdResult	Other