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A complimentary benzophenone cross-linking/mass spectrometry photochemistry

ABSTRACT: We introduce a complimentary, heterobifunctional, photoactivatable, benzophenone containing cross-linker and show its successful application to cross-linking/mass spectrometry, by increasing data density, when used alongside a previously developed diazirine-based heterobifunctional cross-linker.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Adam Belsom

LAB HEAD: Juri Rappsilber

PROVIDER: PXD004920 | Pride | 2017-04-26

REPOSITORIES: Pride

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			Action	DRS
	Belsom_Rappsilber_sulfoSBP.csv	Csv
	Belsom_Rappsilber_sulfoSBP_01.raw	Raw
	Belsom_Rappsilber_sulfoSBP_02.raw	Raw
	Belsom_Rappsilber_sulfoSBP_03.raw	Raw
	Belsom_Rappsilber_sulfoSBP_04.raw	Raw

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Complementary Benzophenone Cross-Linking/Mass Spectrometry Photochemistry.

Belsom Adam A Mudd Gemma G Giese Sven S Auer Manfred M Rappsilber Juri J

Analytical chemistry 20170504 10

Use of a heterobifunctional photoactivatable cross-linker, sulfo-SDA (diazirine), has yielded high-density data that facilitated structure modeling of individual proteins. We expand the photoactivatable chemistry toolbox here with a second reagent, sulfo-SBP (benzophenone). This further increases the density of photo-cross-linking to a factor of 20× over conventional cross-linking. Importantly, the two different photoactivatable groups display orthogonal directionality, enabling access to differ ...[more]

PMID: 28430416

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