Proteomics

Dataset Information

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Identification of novel RHOA interacting patners


ABSTRACT: RHO subfamily of small GTPases comprise highly conserved family members RHOA, RHOB, and RHOC which cycle between GTP-bound 'active' and GDP-bound 'inactive' states. In the active form, RHO proteins interact with a variety of downstream effector proteins, controlling their activity and function. Many of the RHO subfamily effector proteins such as ROCK, PKN, and mDIA, are key regulators of actin cytoskeleton and cell motility. To identify novel effector proteins for RHOA,we carried out a GST-pulldown from heavy or light SILAC labelled HeLa cells using GST tagged GTP-bound RHOA, or GST alone control, as bait. Pulldowns were performed in duplicates with switched labellings. Specific interactors were destinguished from the background on the basis of SILAC Heavy to Light ratios between GST-RHOA and GST alone pulldowns.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Faraz Mardakheh  

LAB HEAD: Christopher J Marshall

PROVIDER: PXD004934 | Pride | 2016-11-01

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20150613_Faraz_gel_lane1_1.raw Raw
20150613_Faraz_gel_lane1_2.raw Raw
20150613_Faraz_gel_lane1_3.raw Raw
20150613_Faraz_gel_lane1_4.raw Raw
20150613_Faraz_gel_lane1_5.raw Raw
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