Proteomics

Dataset Information

0

Identification of Cdc14 phopho-targets in response to DNA damage

ABSTRACT: We have previously shown that the Cdc14 phosphatase is essential for an efficient repair of a DNA lesion, however we still missing how the phosphatase exerts this molecular functions and what are its targets during the repair process. To identify Cdc14 phospho-targets in response to DNA damage, we performed mass spectrometry analysis of Wild-type and Cdc14 deficient cells before and after inducing a single DSBs by expressing the HO endonuclease. Wild-type and a thermosensitive allele cdc14-1 were grown overnight and blocked in G2/M by using nocodazole to avoid cell cycle-dependent changes in protein phosphorylation between both strains. After the block was attained, cells were transfer to 37C to deplete Cdc14 activity prior HO induction. By, using this approach we have screened for proteins containing quantitate low levels of phosphorylated residues after the induction of the DNA lesion that occurs only when Cdc14 is active.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Culture

SUBMITTER: Peter Faull  

LAB HEAD: Peter Allen Faull

PROVIDER: PXD004966 | Pride | 2016-12-22

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
F011807.dat Other
F011807.mgf Mgf
F011807.mzid Mzid
F011808.dat Other
F011808.mgf Mgf
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Publications

Stabilization of the metaphase spindle by Cdc14 is required for recombinational DNA repair.

Villoria María Teresa MT   Ramos Facundo F   Dueñas Encarnación E   Faull Peter P   Cutillas Pedro Rodríguez PR   Clemente-Blanco Andrés A  

The EMBO journal 20161116 1

Cells are constantly threatened by multiple sources of genotoxic stress that cause DNA damage. To maintain genome integrity, cells have developed a coordinated signalling network called DNA damage response (DDR). While multiple kinases have been thoroughly studied during DDR activation, the role of protein dephosphorylation in the damage response remains elusive. Here, we show that the phosphatase Cdc14 is essential to fulfil recombinational DNA repair in budding yeast. After DNA double-strand b  ...[more]

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