Dataset Information

Quantitative proteomics of bloodstream infection

ABSTRACT: We have performed LC-MS based plasma proteomics of twenty febrile patients coming in at Peijas Hospital, Helsinki University Hospital (Vantaa, Finland). At the same time, blood culture was performed to classify the patients to blood culture positive and negative for bloodstream infections. Blood culture negative patients served as controls for the positive ones and these two groups were used for identifying protein profile changes occurring in positive patients. We have subsequently performed various statistical analyses such as principal component analysis as well as pathway analysis by two different methods. We show, in the current study, proteins significantly differing in these two groups as well as dysregulated pathways. These changes seem not to depend on bacterial species as different patients had different blood stream infections. Further, we statistically analyzed the proteomic dataset to find the potential biomarkers which can classify the two classes of patients.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Sakari Joenväärä

LAB HEAD: Risto Renkonen

PROVIDER: PXD005022 | Pride | 2017-02-28

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20151120_KUUSELA_01a.mzXML	Mzxml
	20151120_KUUSELA_01b.mzXML	Mzxml
	20151120_KUUSELA_01c.mzXML	Mzxml
	20151120_KUUSELA_02a.mzXML	Mzxml
	20151120_KUUSELA_02b.mzXML	Mzxml

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Publications

Changes in plasma protein levels as an early indication of a bloodstream infection.

Kuusela Pentti P Saraswat Mayank M Joenväärä Sakari S Kaartinen Johanna J Järvinen Asko A Renkonen Risto R

PloS one 20170224 2

Blood culture is the primary diagnostic test performed in a suspicion of bloodstream infection to detect the presence of microorganisms and direct the treatment. However, blood culture is slow and time consuming method to detect blood stream infections or separate septic and/or bacteremic patients from others with less serious febrile disease. Plasma proteomics, despite its challenges, remains an important source for early biomarkers for systemic diseases and might show changes before direct evi ...[more]

PMID: 28235076

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