Dataset Information

A quantitative map of proteome dynamics during neuronal differentiation

ABSTRACT: Neuronal differentiation is a multistep process that shapes and re-shapes neurons by progressing through several typical stages, including axon outgrowth, dendritogenesis and synapse formation. To systematically profile protein expression during neuronal differentiation we have used cultured hippocampal neurons at different developmental stages in combination with triplex stable isotope dimethyl labeling technique coupled to high-resolution tandem mass spectrometry (LC-MS/MS). In total >1,500 proteins show more than two-fold expression changes during the different developmental steps, indicating extensive remodeling of the neuron proteome during differentiation. To demonstrate the strength of our resource, we focused on neural cell adhesion molecule 1 (NCAM1) as a regulator for dendritic outgrowth during neuronal development. The transmembrane isoform of NCAM1, NCAM180, is strongly upregulated during dendrite outgrowth, highly enriched in dendritic growth cones and interacts with a large variety of actin binding proteins. Inducing actin polymerization rescues the NCAM1 knockdown phenotype, suggesting that NCAM180 stimulates dendritic arbor development by promoting actin filament growth at the dendritic growth cone. Thus, our quantitative map of neuronal proteome dynamics will serve as a rich resource for further analyses of neurodevelopmental regulated processes.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain, Cell Culture

SUBMITTER: Christian Frese

LAB HEAD: AF Maarten Altelaar

PROVIDER: PXD005031 | Pride | 2017-01-17

REPOSITORIES: Pride

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Quantitative Map of Proteome Dynamics during Neuronal Differentiation.

Frese Christian K CK Mikhaylova Marina M Stucchi Riccardo R Gautier Violette V Liu Qingyang Q Mohammed Shabaz S Heck Albert J R AJR Altelaar A F Maarten AFM Hoogenraad Casper C CC

Cell reports 20170201 6

Neuronal differentiation is a multistep process that shapes and re-shapes neurons by progressing through several typical stages, including axon outgrowth, dendritogenesis, and synapse formation. To systematically profile proteome dynamics throughout neuronal differentiation, we took cultured rat hippocampal neurons at different developmental stages and monitored changes in protein abundance using a combination of stable isotope labeling and high-resolution liquid chromatography-tandem mass spect ...[more]

PMID: 28178528

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			Action	DRS
	CF_neuron_late_rat1.msf	Msf
	CF_neuron_late_rat2.msf	Msf
	DIV1_5_14_rat1.zip	Other
	DIV1_5_14_rat2.zip	Other
	MSF_IP.zip	Other