Dataset Information

Comparative proteome of the BeWo and HTR8/SVneo trophoblastic cell lines

ABSTRACT: Here, we have undertaken comparative label-free quantitative (LFQ) proteomic profiling of the proteins expressed in BeWo and HTR8/SVneo cell lines using a mass spectrometry approach. We have identified 1557 proteins in total. 915 proteins were expressed in both cell lines including various transcription factors, chaperones and transport proteins. A further 338 and 304 proteins were uniquely expressed in BeWo and HTR8/SVneo cells respectively. Our bioinformatics analysis reflects the known epithelial and mesenchymal phenotypes of these cell models with principal differences in GO observed in ‘Cell junction’, ‘Catenin complex’ as well as ‘Cell adhesion’ and ‘Cell differentiation’. Our novel comparative proteomic analysis of these trophoblastic cell lines will set the stage for the use of trophoblastic cell lines for the study of pregnancy disorders and further research focusing on placental function.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Paulina Szklanna

LAB HEAD: Patricia B. Maguire

PROVIDER: PXD005045 | Pride | 2018-07-05

REPOSITORIES: Pride

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Comparative proteomic analysis of trophoblast cell models reveals their differential phenotypes, potential uses, and limitations.

Szklanna Paulina B PB Wynne Kieran K Nolan Marie M Egan Karl K Áinle Fionnuala Ní FN Maguire Patricia B PB

Proteomics 20170501 10

Trophoblastic cell lines are widely used in in vitro studies of placental function as a surrogate for primary trophoblasts. To date, no reference proteomics dataset exists to directly compare the shared and unique characteristics of these cells. Here, we performed comparative proteomic profiling of the BeWo and HTR8/SVneo cell lines using label-free quantitative MS. A total of 1557 proteins were identified, which included 338 uniquely attributed to BeWo cells, and a further 304 specifically iden ...[more]

PMID: 28317260

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