Dataset Information

Proteomic analysis of Kveim reagent identifies targets of cellular immunity in sarcoidosis

ABSTRACT: Kveim-reagent (Kv) skin testing was a historical method of diagnosing sarcoidosis. Intradermal injection of treated sarcoidosis spleen tissue resulted in a granuloma response at injection site by 4-6 weeks. Previous work indicates proteins as the possible trigger of this reaction. We aimed to identify Kv-specific proteins and characterise the ex vivo response of Peripheral Blood Mononuclear Cells (PBMCs) from sarcoidosis, tuberculosis and healthy control patients when stimulated with both Kv and selected Kv-specific proteins.

INSTRUMENT(S): 6520 Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Spleen

SUBMITTER: Robert Parker

LAB HEAD: Ajit Lalvani

PROVIDER: PXD005150 | Pride | 2017-01-31

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	RawDataKveimMeasurementII-III.zip	Other
	mascot_daemon_merge (F069705).mzid_mascot_daemon_merge_(F069705).pride.mgf.gz	Mzid
	mascot_daemon_merge (F069705).pride.mztab.gz	Mztab
	mascot_daemon_merge (F069706).mzid_mascot_daemon_merge_(F069706).pride.mgf.gz	Mzid
	mascot_daemon_merge (F069706).pride.mztab.gz	Mztab

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Publications

Proteomic Analysis of Kveim Reagent Identifies Targets of Cellular Immunity in Sarcoidosis.

Eberhardt Christian C Thillai Muhunthan M Parker Robert R Siddiqui Nazneen N Potiphar Lee L Goldin Rob R Timms John F JF Wells Athol U AU Kon Onn M OM Wickremasinghe Melissa M Mitchell Donald D Weeks Mark E ME Lalvani Ajit A

PloS one 20170123 1

<h4>Background</h4>Kveim-reagent (Kv) skin testing was a historical method of diagnosing sarcoidosis. Intradermal injection of treated sarcoidosis spleen tissue resulted in a granuloma response at injection site by 4-6 weeks. Previous work indicates proteins as the possible trigger of this reaction. We aimed to identify Kv-specific proteins and characterise the ex vivo response of Peripheral Blood Mononuclear Cells (PBMCs) from sarcoidosis, tuberculosis and healthy control patients when stimulat ...[more]

PMID: 28114394

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