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LncRNA MALAT1 interactome - Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation

ABSTRACT: Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a long non-coding RNA (lncRNA) that was first discovered as a prognostic marker for lung cancer metastasis. MALAT1 has been implicated in the tumorigenesis of numerous tumor types. To further delineate the underlying molecular mechanism, we established a high-throughput strategy to characterize the interacting proteins of MALAT1 by combining RNA pull down, quantitative proteomics, bioinformatics analysis, and experimental validation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Cell Culture

DISEASE(S): Hepatocellular Carcinoma

SUBMITTER: Ruibing Chen

LAB HEAD: Ruibing Chen

PROVIDER: PXD005154 | Pride | 2018-10-26

REPOSITORIES: Pride

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			Action	DRS
	MALAT1_1-01.msf	Msf
	MALAT1_1.msf	Msf
	MALAT1_10_1.raw	Raw
	MALAT1_10_2.raw	Raw
	MALAT1_1_1.raw	Raw

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Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.

Chen Ruibing R Liu Yun Y Zhuang Hao H Yang Baicai B Hei Kaiwen K Xiao Mingming M Hou Chunyu C Gao Huajun H Zhang Xinran X Jia Chenxi C Li Lingjun L Li Yongmei Y Zhang Ning N

Nucleic acids research 20170901 17

Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a broadly expressed lncRNA involved in many aspects of cellular processes. To further delineate the underlying molecular mechanism, we employed a high-throughput strategy to characterize the interacting proteins of MALAT1 by combining RNA pull-down, quantitative proteomics, bioinformatics, and experimental validation. Our approach identified 127 potential MALAT1-interacting proteins and established a highly connected MALAT1 inter ...[more]

PMID: 28973437

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