Dataset Information

GWI Mouse model SIDL - Complementary proteomic approaches reveal mitochondrial dysfunction, immune and inflammatory dysregulation in a mouse model of gulf war illness

ABSTRACT: Long term consequences of combined pyridostigmine bromide and permethrin exposure in C57BL6/J mice using a well characterized mouse model of exposure to these Gulf War agents. Expanding on earlier work, we used orthogonal proteomic approaches to identify pathways that are chronically impacted in the mouse CNS due to semi-acute GW agent exposure early in life. These analyses were performed on soluble and membrane-bound protein fractions from brain samples using two orthogonal isotopic labeling LC-MS/MS proteomic approaches – stable isotope dimethyl labeling (SIDL) and isobaric tags for relative and absolute quantitation (iTRAQ). The use of these approaches allowed for greater coverage of proteins than was possible by either one alone and revealed both distinct and overlapping datasets. This combined analysis identified changes in several mitochondrial, as well as immune and inflammatory pathways after GW agent exposure. The work discussed here provides insight into GW-agent exposure dependent mechanisms that adversely affect mitochondrial function and immune and inflammatory regulation at five months post exposure to PB+PER.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

DISEASE(S): Persian Gulf Syndrome

SUBMITTER: Gogce Crynen

LAB HEAD: Ghania Ait Ghezala

PROVIDER: PXD005198 | Pride | 2017-02-01

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	25Jan2015_ZZ_PBS_20ACN_GROUP1_150125150048.raw	Raw
	25Jan2015_ZZ_PBS_20ACN_GROUP1_150125163740.msf	Msf
	25Jan2015_ZZ_PBS_20ACN_GROUP1_150125163740.raw	Raw
	25Jan2015_ZZ_PBS_20ACN_GROUP2_150125181249.msf	Msf
	25Jan2015_ZZ_PBS_20ACN_GROUP2_150125181249.raw	Raw

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Publications

Complementary proteomic approaches reveal mitochondrial dysfunction, immune and inflammatory dysregulation in a mouse model of Gulf War Illness.

Zakirova Zuchra Z Reed Jon J Crynen Gogce G Horne Lauren L Hassan Samira S Mathura Venkatarajan V Mullan Michael M Crawford Fiona F Ait-Ghezala Ghania G

Proteomics. Clinical applications 20170512 9-10

<h4>Purpose</h4>Long-term consequences of combined pyridostigmine bromide (PB) and permethrin (PER) exposure in C57BL6/J mice using a well-characterized mouse model of exposure to these Gulf War (GW) agents were explored at the protein level.<h4>Experimental design</h4>We used orthogonal proteomic approaches to identify pathways that are chronically impacted in the mouse CNS due to semiacute GW agent exposure early in life. These analyses were performed on soluble and membrane-bound protein frac ...[more]

PMID: 28371386

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