Proteomics

Dataset Information

0

Proteomic profiles of synovial fluids derived from autologous chondrocyte implantation surgery


ABSTRACT: The pathology of failure in currently used biological treatments (cell therapies and microfracture) for cartilage repair or early osteoarthritis (OA) is poorly understood. We aimed to identify a reliable panel of biological predictors, present in synovial fluid, which can be used in the preoperative setting to optimise patient selection and therapy efficacy. The-long term aim is to reduce the number of patients who progress to end-stage OA and require knee replacement by making earlier, less invasive treatments more effective. In this first stage, we have taken synovial fluid aspirates from patients undergoing autologous chondrocyte implantation (ACI) at our centre in Oswestry, UK. Synovial fluids (SFs) were obtained from 14 ACI responders (mean Lysholm improvement of 33 (17-54)) and 13 non-responders (mean Lysholm decrease of 14 (4-46)) at the two stages of surgery (cartilage harvest and chondrocyte implantation). Dynamic range compression of synovial fluids coupled with label-free quantification mass spectrometry (MS), was used in an unbiased approach to identify predictive markers of ACI success or failure and to investigate the biological pathways involved in the clinical response to ACI.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Synovial Tissue

DISEASE(S): Osteoarthritis

SUBMITTER: Emma Wilson  

LAB HEAD: Mandy Peffers

PROVIDER: PXD005220 | Pride | 2017-07-04

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
220216_LF10_5fold_2h.mzid.gz Mzid
220216_LF10_5fold_2h.pride.mztab.gz Mztab
220216_LF11_5fold_2h.mzid.gz Mzid
220216_LF11_5fold_2h.pride.mztab.gz Mztab
220216_LF12_5fold_2h.mgf Mgf
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Publications

Autologous chondrocyte implantation-derived synovial fluids display distinct responder and non-responder proteomic profiles.

Hulme Charlotte H CH   Wilson Emma L EL   Peffers Mandy J MJ   Roberts Sally S   Simpson Deborah M DM   Richardson James B JB   Gallacher Pete P   Wright Karina T KT  

Arthritis research & therapy 20170630 1


<h4>Background</h4>Autologous chondrocyte implantation (ACI) can be used in the treatment of focal cartilage injuries to prevent the onset of osteoarthritis (OA). However, we are yet to understand fully why some individuals do not respond well to this intervention. Identification of a reliable and accurate biomarker panel that can predict which patients are likely to respond well to ACI is needed in order to assign the patient to the most appropriate therapy. This study aimed to compare the base  ...[more]

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