Proteomics

Dataset Information

0

Chemical cross-linking of the Spindly-RZZ complex followed by mass spectrometry


ABSTRACT: Kinetochores are macromolecular assemblies that connect chromosomes to spindle microtubules during mitosis. The metazoan-specific ≈800 kilodalton ROD-Zwilch-ZW10 (RZZ) complex builds a fibrous corona that assembles on mitotic kinetochores prior to microtubule attachment to promote chromosome alignment and robust spindle assembly checkpoint signaling. Here, we combine biochemical reconstitutions, single particle electron cryo microscopy, cross-linking mass spectrometry, and structural modeling to build a complete model of human RZZ

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Victor Solis  

LAB HEAD: Andrea Musacchio

PROVIDER: PXD005287 | Pride | 2022-02-28

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
AG_Herzog_herzog_E1303_296.raw Raw
AG_Herzog_herzog_E1303_297.raw Raw
AG_Herzog_herzog_E1303_299.raw Raw
AG_Herzog_herzog_E1303_300.raw Raw
AG_Herzog_herzog_E1303_302.raw Raw
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