Ontology highlight
ABSTRACT:
INSTRUMENT(S): TripleTOF 5600
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER: Yang Wang
LAB HEAD: Qing-Yu He
PROVIDER: PXD005291 | Pride | 2022-02-25
REPOSITORIES: Pride
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20150709_3B_4.wiff | Wiff |
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Lu Shaohua S Zhang Jing J Lian Xinlei X Sun Li L Meng Kun K Chen Yang Y Sun Zhenghua Z Yin Xingfeng X Li Yaxing Y Zhao Jing J Wang Tong T Zhang Gong G He Qing-Yu QY
Nucleic acids research 20190901 15
It has been a long debate whether the 98% 'non-coding' fraction of human genome can encode functional proteins besides short peptides. With full-length translating mRNA sequencing and ribosome profiling, we found that up to 3330 long non-coding RNAs (lncRNAs) were bound to ribosomes with active translation elongation. With shotgun proteomics, 308 lncRNA-encoded new proteins were detected. A total of 207 unique peptides of these new proteins were verified by multiple reaction monitoring (MRM) and ...[more]