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Comparative proteomics analysis of microalgae Prototheca associated with severe, mild and non-infectious strains

ABSTRACT: Three strains, P. zopfii genotype 1 (SAG 2063), P. zopfii genotype 2 (SAG 2021) and P. blaschkeae (SAG 2064) from the culture collection at the Institute of Animal Hygiene and Environmental Health, Freie Universität Berlin, Germany were cultured six times independently at 37°C in Sabouraud dextrose liquid medium until OD600 measurement reached 0.6 to 0.9. Following the cell harvest by brief centrifugation, the cells were lysed and label free quantitative proteomics analysis were carried out to investigate the differences between these strains.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Prototheca Zopfii

TISSUE(S): Plant Cell

DISEASE(S): Mastitis

SUBMITTER: Jayaseelan Murugaiyan

LAB HEAD: Jayaseelan Murugaiyan

PROVIDER: PXD005305 | Pride | 2017-01-02

REPOSITORIES: Pride

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	III_3_04.raw	Raw
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Publications

Label-Free Quantitative Proteomic Analysis of Harmless and Pathogenic Strains of Infectious Microalgae, Prototheca spp.

Murugaiyan Jayaseelan J Eravci Murat M Weise Christoph C Roesler Uwe U

International journal of molecular sciences 20161229 1

Microalgae of the genus Prototheca (P.) spp are associated with rare algal infections of invertebrates termed protothecosis. Among the seven generally accepted species, P. zopfii genotype 2 (GT2) is associated with a severe form of bovine mastitis while P. blaschkeae causes the mild and sub-clinical form of mastitis. The reason behind the infectious nature of P. zopfii GT2, while genotype 1 (GT1) remains non-infectious, is not known. Therefore, in the present s ...[more]

PMID: 28036087

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