Proteomics

Dataset Information

0

Targeting the Scaffold Protein Plectin by an Organometallic as a Strategy to Inhibit Tumour Invasiveness- cytoplasmic proteins of arsenic trioxide treated HCT 116 epithelial cells, 3h

ABSTRACT: The inability of determining direct molecular targets of metal-based therapeutic agents beyond the platinum class is one of the major bottlenecks in their development. Here, we employ an integrated chemical proteomics and response profiling approach to identify and validate plestatins, based on an organometallic ruthenium(II) scaffold, as selective plectin-targeting agents. Plectin is a giant scaffold protein involved in controlling cytoarchitecture. Subcytotoxic concentrations of plestatins induce drastic morphological phenotypes on the microtubule (MT) network accompanied by a global down-regulation of translational activity. Plestatins possess anti-invasive properties in tumour spheroids and also display tumour-inhibiting effects in vivo after oral administration. The ability of plestatins to alter the MT network via plectin represents a unique mechanism to target MTs and shows promise as an anticancer strategy.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hct-116 Cell, Colon

SUBMITTER: Christopher Gerner  

LAB HEAD: Christopher Gerner

PROVIDER: PXD005369 | Pride | 2022-03-01

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
HCT116_profiling_cyt_arsenic_trioxide_3h_1_1.mgf Mgf
HCT116_profiling_cyt_arsenic_trioxide_3h_1_1.mzid.gz Mzid
HCT116_profiling_cyt_arsenic_trioxide_3h_1_1.pride.mgf.gz Mgf
HCT116_profiling_cyt_arsenic_trioxide_3h_1_1.pride.mztab.gz Mztab
HCT116_profiling_cyt_arsenic_trioxide_3h_1_1.raw Raw
Items per page:
1 - 5 of 30
altmetric image

Publications

Organometallic metal(arene) anticancer agents require ligand exchange for their anticancer activity and this is generally believed to confer low selectivity for potential cellular targets. However, using an integrated proteomics-based target-response profiling approach as a potent hypothesis-generating procedure, we found an unexpected target selectivity of a ruthenium(arene) pyridinecarbothioamide (plecstatin) for plectin, a scaffold protein and cytolinker, which was validated in a plectin knoc  ...[more]

Similar Datasets

Proteomics Targeting the Scaffold Protein Plectin by an Organometallic as a Strategy to Inhibit Tumour Invasiveness - cytoplasmic proteins of untreated HCT 116 epithelial cells, 24h
2022-03-01 | PXD005373 | Pride
Proteomics Targeting the Scaffold Protein Plectin by an Organometallic as a Strategy to Inhibit Tumour Invasiveness- cytoplasmic proteins of untreated HCT 116 epithelial cells, 3h
2022-03-01 | PXD005367 | Pride
Proteomics Targeting the Scaffold Protein Plectin by an Organometallic as a Strategy to Inhibit Tumour Invasiveness- nuclear proteins of untreated HCT 116 epithelial cells, 3h
2022-03-01 | PXD005370 | Pride
Proteomics Targeting the Scaffold Protein Plectin by an Organometallic as a Strategy to Inhibit Tumour Invasiveness- cytoplasmic proteins of plestatins-2 treated HCT 116 epithelial cells, 3h
2022-03-01 | PXD005368 | Pride
Proteomics Targeting the Scaffold Protein Plectin by an Organometallic as a Strategy to Inhibit Tumour Invasiveness - nuclear proteins of untreated HCT 116 epithelial cells, 24h
2022-03-01 | PXD005375 | Pride
Proteomics Targeting the Scaffold Protein Plectin by an Organometallic as a Strategy to Inhibit Tumour Invasiveness- cytoplasmic proteins of plestatins-2 treated HCT 116 epithelial cells, 24h
2022-03-01 | PXD005374 | Pride
Proteomics Targeting the Scaffold Protein Plectin by an Organometallic as a Strategy to Inhibit Tumour Invasiveness- nuclear proteins of plestatins-2 treated HCT 116 epithelial cells, 24h
2022-03-01 | PXD005376 | Pride
Proteomics Targeting the Scaffold Protein Plectin by an Organometallic as a Strategy to Inhibit Tumour Invasiveness- nuclear proteins of plestatins-2 treated HCT 116 epithelial cells, 3h
2022-03-01 | PXD005371 | Pride
Proteomics Targeting the Scaffold Protein Plectin by an Organometallic as a Strategy to Inhibit Tumour Invasiveness- nuclear proteins of arsenic trioxide treated HCT 116 epithelial cells, 3h
2022-03-01 | PXD005372 | Pride
Proteomics Targeting the Scaffold Protein Plectin by an Organometallic as a Strategy to Inhibit Tumour Invasiveness- whole cell lysate of HCT 116 epithelial cells, plestatins-3 normal pull down 19h
2022-03-01 | PXD005387 | Pride