Proteomics

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Composition and immune environment of urinary bladder matrix scaffolds


ABSTRACT: Urinary bladder matrix (UBM) is used clinically for treatment of diabetic ulcers, abdominal wall reconstruction, soft tissue filling among other applications. Composed mainly of collagen, the material is a mix of fibrous collagen structures and sheet-forming structures characteristic of the urinary bladder matrix basal lamina. Upon application of a biomaterial in a traumatic or non-traumatic setting, there is a cascade of immune cells that react to the damaged tissue and biomaterial. Here, through the use of multicolor flow cytometry, we describe the various cells that infiltrate the UBM scaffold in subcutaneous and volumetric muscle injury model. A wide variety of immune cells are found in the UBM scaffold immune microenvironment (SIM) including F4/80 + macrophages, CD11c + dendritic cells, CD3 + T cells and CD19 + B cells. UBM induced a systemic IL-4 upregulation and a local M2-macrophage response. The recruitment and activation of these cells is dependent upon signals from the scaffold and communication between the different cell types present.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

SUBMITTER: Akhilesh Pandey  

LAB HEAD: Jennifer H. Elisseeff

PROVIDER: PXD005400 | Pride | 2017-06-09

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
ECM_260.raw Raw
ECM_42.raw Raw
ECM_48.raw Raw
ECM_BMMM.raw Raw
Matristem_260_rep2_spike.raw Raw
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Publications

Proteomic composition and immunomodulatory properties of urinary bladder matrix scaffolds in homeostasis and injury.

Sadtler Kaitlyn K   Sommerfeld Sven D SD   Wolf Matthew T MT   Wang Xiaokun X   Majumdar Shoumyo S   Chung Liam L   Kelkar Dhanashree S DS   Pandey Akhilesh A   Elisseeff Jennifer H JH  

Seminars in immunology 20170201


Urinary bladder matrix (UBM) is used clinically for management of wounds and reinforcement of surgical soft tissue repair, among other applications. UBM consists of the lamina propria and basal lamina of the porcine urinary bladder, and is decellularized as part of the process to manufacture the medical device. UBM is composed mainly of Collagen I, but also contains a wide variety of fibrillar and basement membrane collagens, glycoproteins, proteoglycans and ECM-associated factors. Upon applicat  ...[more]

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