Dataset Information

Comparative (phospho)proteomic profiling of wheat shoots from different cultivars

ABSTRACT: Wheat is a cereal grain and one of the world’s major food crops. Recent advances in wheat genome sequencing are by now facilitating genomic and proteomic analyses of this crop. However, little is known about the protein levels of hexaploid versus tetraploid wheat cultivars, and knowledge on phosphorylated proteins still limited. Using our recently established (phospho)proteomic workflow, we performed a parallel analysis of the proteome and phosphoproteome on seedling leaves from two hexaploid wheat cultivars (Pavon 76 and USU-Apogee) and a tetraploid wheat (Senatore Cappelli). This revealed that a large portion of proteins and phosphosites can be quantified in all cultivars. Our shotgun proteomics data revealed a high similarity between hexaploid and tetraploid varieties with respect to protein abundance. However, we could identify a set of proteins that were differentially abundant between hexaploid and tetraploid cultivars. In addition, already at seedling stage, a small set of proteins were differential between the small (USU-Apogee) and larger hexaploid wheat cultivar (Pavon 76), which could potentially act as growth predictors. Finally, the phosphosites identified in this study can be retrieved from the in-house developed plant PTM-Viewer (bioinformatics.psb.ugent.be/webtools/ptm_viewer/), making this the first repository for phosphorylated wheat proteins. This paves the way for further in depth, quantitative (phospho)proteome-wide differential analyses upon a specific trigger or environmental change.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Triticum Aestivum (wheat) Triticum Turgidum Subsp. Durum

TISSUE(S): Plant Cell, Shoot

SUBMITTER: Lam Dai Vu

LAB HEAD: Ive De Smet

PROVIDER: PXD005437 | Pride | 2017-04-03

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	E09207_2p_4880_Elisabeth_Dai_phospho_Apogee1.raw	Raw
	E09207_2p_4880_Elisabeth_Dai_phospho_Apogee1_repl2.raw	Raw
	E09208_1p_4880_Elisabeth_Dai_NP_Apogee1.raw	Raw
	E09208_1p_4880_Elisabeth_Dai_NP_Apogee1_repl2.raw	Raw
	E09209_2p_4880_Elisabeth_Dai_phospho_Apogee2.raw	Raw

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Publications

Proteome Profiling of Wheat Shoots from Different Cultivars.

Vu Lam Dai LD Verstraeten Inge I Stes Elisabeth E Van Bel Michiel M Coppens Frederik F Gevaert Kris K De Smet Ive I

Frontiers in plant science 20170313

Wheat is a cereal grain and one of the world's major food crops. Recent advances in wheat genome sequencing are by now facilitating its genomic and proteomic analyses. However, little is known about possible differences in total protein levels of hexaploid versus tetraploid wheat cultivars, and also knowledge of phosphorylated wheat proteins is still limited. Here, we performed a detailed analysis of the proteome of seedling leaves from two hexaploid wheat cultivars (<i>Triticum aestivum</i> L. ...[more]

PMID: 28348574

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