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Quantitative proteomic profiling of fibroblasts in oesophageal adenocarcinoma

ABSTRACT: The accumulatiob of cancer-associated fibroblasts in tumours correlates with poor prognosis across cancer types including oesophageal adenocarcinoma. In this study we examined the proteomic profile of cancer-associated fibroblasts compared to normal fibroblasts.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Antigoni Manousopoulou

LAB HEAD: Spiros D. Garbis

PROVIDER: PXD005444 | Pride | 2018-08-13

REPOSITORIES: Pride

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			Action	DRS
	01APR16_cafnof.pep.xml	Pepxml
	01APR16_cafnof.prot.xml	Xml
	11jan16_caf_nof_10.raw	Raw
	11jan16_caf_nof_11.raw	Raw
	11jan16_caf_nof_12.raw	Raw

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Publications

Quantitative proteomic profiling of primary cancer-associated fibroblasts in oesophageal adenocarcinoma.

Manousopoulou Antigoni A Hayden Annette A Mellone Massimiliano M Garay-Baquero Diana J DJ White Cory H CH Noble Fergus F Lopez Monette M Thomas Gareth J GJ Underwood Timothy J TJ Garbis Spiros D SD

British journal of cancer 20180329 9

<h4>Background</h4>Cancer-associated fibroblasts (CAFs) form the major stromal component of the tumour microenvironment (TME). The present study aimed to examine the proteomic profiles of CAFs vs. normal fibroblasts (NOFs) from patients with oesophageal adenocarcinoma to gain insight into their pro-oncogenic phenotype.<h4>Methods</h4>CAFs/NOFs from four patients were sub-cultured and analysed using quantitative proteomics. Differentially expressed proteins (DEPs) were subjected to bioinformatics ...[more]

PMID: 29593339

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Project description:Dll4-Notch signaling is required for cell fate decisions and neoplasias. However, emerging evidence suggests a role for Dll4-Notch signaling in metabolic and immune diseases. To date, there is no evidence of a direct effect of Dll4-Notch signaling inhibition on pancreatic islet function and insulin secretion.

2018-08-13 | PXD005333 | Pride

Regionalized cell and gene signatures govern oesophageal epithelial homeostasis.

Project description:Regionalized disease prevalence is a common feature of the gastrointestinal tract. Herein, we employed regionally resolved Smart-seq3 single-cell sequencing, generating a comprehensive cell atlas of the adult oesophagus. Characterizing the oesophageal axis, we unveil non-uniform distribution of epithelial basal cells, fibroblasts and immune cells. In addition, we reveal a position-dependent, but cell subpopulation-independent, transcriptional signature, collectively generating a regionalized landscape. Combining in vivo models with organoid co-cultures, we demonstrate that proximal and distal basal progenitor cell states are functionally distinct. We find that proximal fibroblasts are more permissive for organoid growth compared to distal fibroblasts and that the immune cell profile is regionalized in two dimensions, where proximal-distal and epithelial-stromal gradients impact epithelial maintenance. Finally, we predict and verify how WNT-, BMP-, IGF- and NRG-signalling are differentially engaged along the oesophageal axis. We establish a cellular and transcriptional framework for understanding oesophageal regionalization, providing a functional basis for epithelial disease susceptibility.

2024-02-22 | GSE254855 | GEO

Genome-Wide DNA Methylation Profiling of Oesophageal Cancer-Associated Myofibroblasts

Project description:The Illumina Infinium HumanMethylation450 BeadChip arrays were performed on a collection of primary oesophageal cancer-associated myofibroblasts (CAM) and their patient-matched adjacent tissue myofibroblasts (ATM). CAM and ATM samples were obtained from patients with oesophageal adenocarcinomas undergoing cancer surgery.

2019-01-10 | GSE97687 | GEO

MiR-193b promotes autophagy and non-apoptotic cell death in oesophageal cancer cells

Project description:Background: Successful treatment of oesophageal cancer is hampered by recurrent drug resistant disease. We have previously demonstrated the importance of apoptosis and autophagy for the recovery of oesophageal cancer cells following drug treatment. When apoptosis (with autophagy) is induced, these cells are chemosensitive and will not recover following chemotherapy treatment. In contrast, when cancer cells exhibit only autophagy and limited Type II cell death, they are chemoresistant and recover following drug withdrawal. Methods: MicroRNA (miRNA) expression profiling of an oesophageal cancer cell line panel was used to identify miRNAs that were important in the regulation of apoptosis and autophagy. The effects of miRNA overexpression on cell death mechanisms and recovery were assessed in the chemoresistant (autophagy inducing) KYSE450 oesophageal cancer cells. Results: MiR-193b was the most differentially expressed miRNA between the chemosensitive and chemoresistant cell lines with higher expression in chemosensitive apoptosis inducing cell lines. Colony formation assays showed that overexpression of miR-193b significantly impedes the ability of KYSE450 cells to recover following 5-fluorouracil (5-FU) treatment. The critical mRNA targets of miR-193b are unknown but target prediction and siRNA data analysis suggest that it may mediate some of its effects through stathmin 1 regulation. Apoptosis was not involved in the enhanced cytotoxicity. Overexpression of miR- 193b in these cells induced autophagic flux and non-apoptotic cell death. Conclusion: These results highlight the importance of miR-193b in determining oesophageal cancer cell viability and demonstrate an enhancement of chemotoxicity that is independent of apoptosis induction.

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Gene induction by low pH in oesophageal cells

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SNORA42 Promote Oesophageal Squamous Cell Carcinoma Development through interacting with DHX9

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RNAseq of Oesophageal Adenocarcinoma Cell Lines

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Expression data from OE33 oesophageal adenocarcinoma tumour cells following 24 hour co-culture with human adipocytes or control M199 medium.

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Expression data from OE33 oesophageal adenocarcinoma tumour cells following 24 hour co-culture with human adipose tissue explants or control M199 medium.

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Circulating serum miRNAs as potential diagnostic biomarkers for oesophageal adenocarcinoma

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2017-10-22 | GSE63108 | GEO

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