Proteomics

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Human ccRCC cell line 786-O with PHD3 knockdown LC-MS/MS

ABSTRACT: Appreciating the pseudohypoxic signalling in ccRCC cells we decided to look into the effects of an established hypoxia-related enzyme, namely PHD3, in ccRCC development. Interestingly, these cells express PHD3 in unusually high amounts, a phenomena yet unexplained. We were interested in determining how ccRCC cells expressing elevated levels of PHD3 would respond to its knockdown. It has been shown that PHD3 has many other functions in addition to its given role as regulating HIF-1 by targeting it for proteosomal degradation. Motivated by these varied roles of PHD3 we decided to look for indications of a systemic-level changes that might be governed by PHD3 in ccRCC. To this end we chose a discovery proteomics –approach to see how ccRCC cells would respond on proteome level to PHD3 depletion.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Renal Clear Cell Carcinoma

SUBMITTER: Petra Miikkulainen  

LAB HEAD: Panu M. Jaakkola

PROVIDER: PXD005453 | Pride | 2017-07-10

REPOSITORIES: pride

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Dataset's files

Source:
Action DRS
Exp1_786O_hox_scr.msf Msf
Exp1_786O_hox_scr.raw Raw
Exp1_786O_hox_siphd3.msf Msf
Exp1_786O_hox_siphd3.raw Raw
Exp1_786O_nox_scr.msf Msf
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Publications

HIF prolyl hydroxylase PHD3 regulates translational machinery and glucose metabolism in clear cell renal cell carcinoma.

Miikkulainen Petra P   Högel Heidi H   Rantanen Krista K   Suomi Tomi T   Kouvonen Petri P   Elo Laura L LL   Jaakkola Panu M PM  

Cancer & metabolism 20170704

<h4>Background</h4>A key feature of clear cell renal cell carcinoma (ccRCC) is the inactivation of the von Hippel-Lindau tumour suppressor protein (pVHL) that leads to the activation of hypoxia-inducible factor (HIF) pathway also in well-oxygenated conditions. Important regulator of HIF-α, prolyl hydroxylase PHD3, is expressed in high amounts in ccRCC. Although several functions and downstream targets for PHD3 in cancer have been suggested, the role of elevated PHD3 expression in ccRCC is not cl  ...[more]

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