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High-flow LC-MS/MS analyses - Conventional-Flow Liquid Chromatography-Mass Spectrometry for Exploratory Bottom-Up Proteomic Analyses

ABSTRACT: The major objective of our study was to find out what are the extra costs and what optimization and adjustment steps of a high-flow LC-MS system have to be undertaken to identify a comparable number of proteins as on a nanoLC-MS system.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Juraj Lenco

LAB HEAD: Jiri Stulik

PROVIDER: PXD005481 | Pride | 2019-11-07

REPOSITORIES: Pride

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			Action	DRS
	JL390_JL382_HeLa_2000ng_01.mgf	Mgf
	JL390_JL382_HeLa_2000ng_01.msf	Msf
	JL390_JL382_HeLa_2000ng_01.raw	Raw
	JL390_JL382_HeLa_2000ng_02.mgf	Mgf
	JL390_JL382_HeLa_2000ng_02.msf	Msf

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Publications

Conventional-Flow Liquid Chromatography-Mass Spectrometry for Exploratory Bottom-Up Proteomic Analyses.

Lenčo Juraj J Vajrychová Marie M Pimková Kristýna K Prokšová Magdaléna M Benková Markéta M Klimentová Jana J Tambor Vojtěch V Soukup Ondřej O

Analytical chemistry 20180404 8

Due to its sensitivity and productivity, bottom-up proteomics based on liquid chromatography-mass spectrometry (LC-MS) has become the core approach in the field. The de facto standard LC-MS platform for proteomics operates at sub-μL/min flow rates, and nanospray is required for efficiently introducing peptides into a mass spectrometer. Although this is almost a "dogma", this view is being reconsidered in light of developments in highly efficient chromatographic columns, and especially with the i ...[more]

PMID: 29582996

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