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Analysis of Trpc6 Phosphorylation in HEK293T cells

ABSTRACT: The aim of this project was to detect phosphorylation on the human calcium Channel TRPC6. TRPC6 is mutated in genetic diseases of the podocyte, a postmitotic cell at the epithelial filtration barrier

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Kidney

SUBMITTER: Markus Rinschen

LAB HEAD: Henning Hagmann

PROVIDER: PXD005482 | Pride | 2017-08-23

REPOSITORIES: Pride

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			Action	DRS
	0011-120830HeHaS01Tscx01pD.RAW	Raw
	0011-120830HeHaS01Tscx01pD_S_Hsa111125.msf	Msf
	0011-120830HeHaS01Tscx02pD.RAW	Raw
	0011-120830HeHaS01Tscx02pD_S_Hsa111125.msf	Msf
	0011-120830HeHaS01Tscx03pD.RAW	Raw

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Publications

Proline-dependent and basophilic kinases phosphorylate human TRPC6 at serine 14 to control channel activity through increased membrane expression.

Hagmann Henning H Mangold Nicole N Rinschen Markus M MM Koenig Tim T Kunzelmann Karl K Schermer Bernhard B Benzing Thomas T Brinkkoetter Paul T PT

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20170906 1

Signaling <i>via</i> the transient receptor potential (TRP) ion channel C6 plays a pivotal role in hereditary and sporadic glomerular kidney disease. Several studies have identified gain-of-function mutations of <i>TRPC6</i> and report induced expression and enhanced channel activity of TRPC6 in association with glomerular diseases. Interfering with TRPC6 activity may open novel therapeutic pathways. TRPC6 channel activity is controlled by protein expression and stability as well as intracellula ...[more]

PMID: 28877958

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