Proteomics

Dataset Information

0

In-depth and reproducibly quantitation of benchmark dataset consiting of 20 files


ABSTRACT: We prepared a benchmark dataset where the various levels of spikeed-in E. Coli proteome that true fold change (i.e. 1 fold, 1.5 fold, 2 fold, 2.5 fold and 3 fold) and true identities of positives/negatives (i.e. E.Coli proteins are true positives while Human proteins are true negatives) are known. To best mimic the proteomics application in comparison of multiple replicates, each fold change

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: XIAOMENG SHEN  

LAB HEAD: JUN QU

PROVIDER: PXD005590 | Pride | 2017-04-20

REPOSITORIES: Pride

Dataset's files

Source:
altmetric image

Publications

An IonStar Experimental Strategy for MS1 Ion Current-Based Quantification Using Ultrahigh-Field Orbitrap: Reproducible, In-Depth, and Accurate Protein Measurement in Large Cohorts.

Shen Xiaomeng X   Shen Shichen S   Li Jun J   Hu Qiang Q   Nie Lei L   Tu Chengjian C   Wang Xue X   Orsburn Benjamin B   Wang Jianmin J   Qu Jun J  

Journal of proteome research 20170525 7


In-depth and reproducible protein measurement in many biological samples is often critical for pharmaceutical/biomedical proteomics but remains challenging. MS1-based quantification using quadrupole/ultrahigh-field Orbitrap (Q/UHF-Orbitrap) holds great promise, but the critically important experimental approaches enabling reliable large-cohort analysis have long been overlooked. Here we described an IonStar experimental strategy achieving excellent quantitative quality of MS1 quantification. Key  ...[more]

Similar Datasets

2018-05-16 | PXD003881 | Pride
2022-01-06 | PXD030780 | Pride
2021-06-24 | PXD017865 | Pride
2021-06-24 | PXD017812 | Pride
2021-06-24 | PXD017915 | Pride
2022-09-10 | GSE212714 | GEO
2024-01-26 | PXD039064 | Pride
2014-02-03 | E-GEOD-30557 | biostudies-arrayexpress
2014-02-03 | GSE30557 | GEO
2022-02-24 | PXD027742 | Pride