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Streptococcus suis SC-19, LC-MSMS

ABSTRACT: Phosphoproteomic analysis to identify deregulated phosphoproteins in stk mutant strain compared to the parental wild type strain SC-19

OTHER RELATED OMICS DATASETS IN: PRJNA347365

INSTRUMENT(S): Q Exactive

ORGANISM(S): Streptococcus Suis

TISSUE(S): Cell Culture

SUBMITTER: Chunyan Zhang

LAB HEAD: Rui Zhou

PROVIDER: PXD005663 | Pride | 2017-03-27

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	P15287_1.raw	Raw
	P15287_150830125233.raw	Raw
	P15287_1_151001084622.raw	Raw
	P15287_2.msf	Msf
	P15287_2.prot.xml	Xml

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Publications

The Eukaryote-Like Serine/Threonine Kinase STK Regulates the Growth and Metabolism of Zoonotic Streptococcus suis.

Zhang Chunyan C Sun Wen W Tan Meifang M Dong Mengmeng M Liu Wanquan W Gao Ting T Li Lu L Xu Zhuofei Z Zhou Rui R

Frontiers in cellular and infection microbiology 20170307

Like eukaryotes, bacteria express one or more serine/threonine kinases (STKs) that initiate diverse signaling networks. The STK from Streptococcus suis is encoded by a single-copy stk gene, which is crucial in stress response and virulence. To further understand the regulatory mechanism of STK in S. suis, a stk deletion strain (Δstk) and its complementary strain (CΔstk) were constructed to systematically decode STK characteristics by applying whole trans ...[more]

PMID: 28326294

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RNA-seq analysis of genes regulated by stk in Streptococcus suis serotype 2

Project description:To reveal the scope of the stk regulation and explore the differences in the regulatory roles of SC-19 and ∆stk, the global gene transcription profiles of the WT and ∆stk strains were determined by RNA-seq analysis.

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The role of Stk in Streptococcus pyogenes

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Project description:Transcriptional profile of Streptococcus pyogenes stk mutant strain JRS2516 vs its wild type parent strain MGAS2221 The RNA prepared from triplicate cultures for the wild type and the stk mutant strains was each labeled with Cy3 and hybridized to duplicate arrays. Reference RNA consisted of pooled RNA for the wild type and stk mutant, labeled with Cy5.

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Comparative Phenotypic, Proteomic, and Phosphoproteomic Analysis Reveals Different Roles of Serine/Threonine Phosphatase and Kinase in the Growth, Cell Division, and Pathogenicity of Streptococcus suis

Project description:To investigate the influence of STP and STK on protein expression in S. suis, we performed a comparative proteomics analysis with the WT, Δstp, and Δstk strains using liquid chromatography mass spectrometry/mass spectrometry (LC-MS/MS).STK and STP are serine/threonine kinases and phosphatases, respectively. Therefore, we next analyzed the changes in the abundance of serine/threonine phosphorylated proteins in Δstp and Δstk compared with the WT strain.

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Project description:RNA-seq analysis of genes regulated by stk in Streptococcus suis serotype 2

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RelA/(p)ppGpp regulates the glucose-starvation induced stringent response in the zoonostic Streptococcus suis

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RelA/(p)ppGpp regulates the glucose-starvation induced stringent response in the zoonostic Streptococcus suis

Project description:Growth curves and (p)ppGpp accumulation assays showed that RelA inactivation could influence S. suis growth and led to incapacity of (p)ppGpp synthesis during glucose starvation. To identify the roles of RelA/(p)ppGpp in global gene regulation in S. suis, we compared the transcriptional profiles of SC-19 [a (p)ppGpp+ strain] and ΔrelA [a (p)ppGpp0 strain during glucose starvation] in both glucose-abundant and -deficient CDM in exponential phase by microarray analysis. A less stringent cut-off limit, 2-fold change, was used. qRT-PCR validation displayed the same trends observed in the microarrays relA mutant strain and its parents strain SC-19 were cultured in both glucose-abundant CDM (CDM containing 1% glucose) and glucose-deficient CDM (CDM containing 2% glucose) respectively. The bacteriain in exponential phase were collected for microarray analysis. Three independent experiments were performed.

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Identification of differential phosphoproteins reveled complex responses of soybean to phosphate starvation through reversible protein phosphorylation

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Next Generation Sequencing Facilitates Quantitative Analysis of N. crassa Wild Type, stk-17ko and stk-17com Transcriptomes treated with or without ketoconazole

Project description:STK-17, a kinase that responses to azoles transcriptionally, is required for azole resistance. By analyzing and comparing the transcriptomes from samples of N. crassa Wild Type, stk-17ko and stk-17com strain treated with or without ketoconazole, the biological processes that would be affected by stk-17 deletion was revealled.

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