Proteomics

Dataset Information

0

Regulation of somatosensory mechanotransduction by Annexin A6


ABSTRACT: Sensory neuron mechanically-activated slowly adapting currents have been linked to noxious mechanosensation. We identified a Conotoxin, Noxious Mechanosensation Blocker -1, that blocks such currents selectively. Using an active biotinylated form of the toxin we identified 67 binding proteins in sensory neurons and sensory neuron-derived cell lines using mass spectrometry. Annexin A6 was the most frequently identified binding protein. Annexin A6 knockout mice showed an enhanced sensitivity to mechanical stimuli. Rapidly adapting currents were enhanced and slowly adapting channels diminished. The percentage of neurons expressing slowly adapting currents fell and non-responsive neurons increased. Conversely, overexpression of annexin A6 in DRG neurons inhibited rapidly adapting currents without effects on slowly adapting currents. Co-expression of annexin A6 with Piezo 2 led to an inhibition of Piezo-mediated rapidly adapting currents. AAV-mediated gene delivery of annexin A6 to sensory neurons in a mouse model of osteoarthritis attenuated mechanical pain. These data demonstrate a role for annexin A6 in somatosensory mechanotransduction.

INSTRUMENT(S): LTQ FT

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Dendritic Cell, Cell Culture

DISEASE(S): Disease Free

SUBMITTER: James Wright  

LAB HEAD: Jyoti Choudhary

PROVIDER: PXD005718 | Pride | 2018-04-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
F011535.mgf Mgf
F011535.mzid.gz Mzid
F011535.pride.mgf.gz Mgf
F011535.pride.mztab.gz Mztab
F018952.mgf Mgf
Items per page:
1 - 5 of 61

Similar Datasets

2019-03-31 | GSE127161 | GEO
2018-08-31 | GSE106526 | GEO
2013-08-28 | GSE46546 | GEO
2013-08-28 | E-GEOD-46546 | biostudies-arrayexpress
2023-11-14 | GSE218063 | GEO
2017-10-01 | GSE102123 | GEO
2023-01-30 | GSE221834 | GEO
2024-03-31 | GSE262604 | GEO
2024-01-31 | GSE236109 | GEO
2009-02-17 | E-MEXP-1718 | biostudies-arrayexpress