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Human Uirnary Extracellular vesicles Proteomics LC-MS

ABSTRACT: A proteomic analysis utilizing UDMSE was performed on exosomes and microvesicle fractions isolated from human urine. more than 2 unique peptides containing proteins were quantified (586). exosomes and microvesicles were compared to each other in a quantitative manner.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine

SUBMITTER: Sakari Joenväärä

LAB HEAD: Risto Renkonen

PROVIDER: PXD005729 | Pride | 2022-03-02

REPOSITORIES: Pride

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			Action	DRS
	20160428_thrombin_exo_01.raw.zip	Raw
	20160428_thrombin_exo_02.raw.zip	Raw
	20160428_thrombin_exo_03.raw.zip	Raw
	20160428_thrombin_exo_04.raw.zip	Raw
	20160428_thrombin_exo_05.raw.zip	Raw

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Publications

N-linked (N-) Glycoproteomics of Urinary Exosomes.

Saraswat Mayank M Joenväära Sakari S Musante Luca L Peltoniemi Hannu H Holthofer Harry H Renkonen Risto R

Molecular & cellular proteomics : MCP 20150801 8

PMID: 26232421

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Quantitative proteomics of bloodstream infection

Project description:We have performed LC-MS based plasma proteomics of twenty febrile patients coming in at Peijas Hospital, Helsinki University Hospital (Vantaa, Finland). At the same time, blood culture was performed to classify the patients to blood culture positive and negative for bloodstream infections. Blood culture negative patients served as controls for the positive ones and these two groups were used for identifying protein profile changes occurring in positive patients. We have subsequently performed various statistical analyses such as principal component analysis as well as pathway analysis by two different methods. We show, in the current study, proteins significantly differing in these two groups as well as dysregulated pathways. These changes seem not to depend on bacterial species as different patients had different blood stream infections. Further, we statistically analyzed the proteomic dataset to find the potential biomarkers which can classify the two classes of patients.

2017-02-28 | PXD005022 | Pride

Differential proteomics of human seminal fluid Normo Vs Astehnozoospermia

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Chronic pancreatitis vs pancreatic cancer LC-MS

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Several proteins are altered in serum of patients having colorectal cancer with variable levels of C-Reactive protein and different survival times

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Comparative serum proteomics of Intraductal papillary mucinous neoplasias and pacreatic adenocarcinoma

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Lung Cancer Exosomes Hypoxic v Normoxic

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C9ORF72 repeat expansion affects the proteome of primary skin fibroblasts in ALS and FTD

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2021-11-02 | PXD023866 | Pride

Project description:Breast milk is a complex liquid that enriched in immunological components and affect the development of the infant immune system. Exosomes, the membranous vesicles of endocytic origin, are ubiquitously in various body fluids which can mediate intercellular communication. MicroRNAs (miRNAs), a well-defined group of non-coding small RNAs, in human breast milk are packaged inside exosomes. Here, we present the identification of miRNAs in human breast milk exosomes using deep sequencing technology. We found that the immune-related miRNAs are enriched in breast milk exosomes, and are resistant to the general harsh conditions. Four small RNA libraries in human breast milk exosomes from four healthy women (30 +/- 0.9 years old, primiparity) when the infant were aged at 60 days were sequenced.

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Project description:Breast milk is the primary source of nutrition for newborns, and rich in immunological components. microRNAs (miRNAs), a well-defined group of non-coding small RNAs, are present in various body fluids (such as breast milk), which are selectively packaged inside the exosomes, a type of membrane vesicles, secreted by most cell types. These exosomal miRNAs could be actively delivered into recipient cells, and regulate target gene expression and recipient cell function. We present the lactation-related miRNA expression profiles in porcine milk exosomes across entire lactation period in pig industry (newborn to 28 days after birth) using deep sequencing technology. We found that the immune-related miRNAs are presented and enriched in breast milk exosomes, and generally resistant to relatively harsh conditions. Notably, these exosomal miRNAs exhibited the higher abundances in the colostrum (newborn to 3 days after birth) than that in the mature milk (7 to 28 days after birth), as well as in the serum of colostrum-feeding piglets compared with the only mature milk-feeding piglets. These immune-related miRNAs-loaded exosomes in breast milk may be transferred into the infant body via the digestive tract. These observations are prelude to the in-depth investigations of the essential roles of the breast milk in the development of the infant’s immune system. Eight small RNA libraries in porcine breast milk exosomes of six lactigenous stages (0, 3, 7, 14, 21 and 28 days after birth) from three female pigs were sequenced.

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High fat diet mice intestinal epithelial exosomal proteins were analyzed by MSMS.

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