Proteomics

Dataset Information

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Purification and characterization of a novel allergenic walnut vicilin-like protein


ABSTRACT: Walnuts are potent inducers of food allergic symptoms. So far, five allergens were identified, among those, Jug r 2 belonging to the vicilin family. Based on the identification of an IgE-reactive clone from a cDNA library in 1999 (Teuber et al.), the sequence fragment of Jug r 2 was determined. However, only limited data is known about the natural form of the protein. Therefore, this study aimed to purify and characterize the natural Jug r 2. During the study we identified another vicilin with both, higher sequence identity to vicilins from related species and IgE cross reactivity as compared to Jug r 2.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Juglans Regia (english Walnut)

TISSUE(S): Plant Cell, Kernel

SUBMITTER: Pawel Dubiela  

LAB HEAD: Pawel Dubiela

PROVIDER: PXD005744 | Pride | 2018-10-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
160304-MClass-16_0655-MELD-AWM.mgf Mgf
160304-MClass-16_0655-MELD-AWM.mzxml Mzxml
160304-MClass-16_0655-MELD-AWM.pride.mgf.gz Mgf
peptides_1_1_0.mzid.gz Mzid
peptides_1_1_0.pride.mztab.gz Mztab
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Publications

Jug r 6 is the allergenic vicilin present in walnut responsible for IgE cross-reactivities to other tree nuts and seeds.

Dubiela Pawel P   Kabasser Stefan S   Smargiasso Nicolas N   Geiselhart Sabine S   Bublin Merima M   Hafner Christine C   Mazzucchelli Gabriel G   Hoffmann-Sommergruber Karin K  

Scientific reports 20180727 1


Walnuts are ranked high in the list of the culprit foods inducing severe allergic reactions. Jug r 2 has been identified as a major allergen in common walnut by cDNA cloning from a somatic cell line. So far, studies were performed on the allergenic activity of recombinant Jug r 2, yet there is still no evidence about the physicochemical characteristics of the natural allergen. Therefore, we aimed to purify and deeply characterize natural Jug r 2 and to assess IgE cross-reactivity among vicilins  ...[more]

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