Proteomics

Dataset Information

0

Comparative proteomic analysis and bioluminescent reporter gene assays to investigate effects of simulated microgravity on Caco-2 cells

ABSTRACT: Microgravity is one of the most important features in spaceflight. Previous evidence has shown that significant changes to the musculoskeletal and immune systems occurred under microgravity. The present study was undertaken to explore the change in protein abundance in human colon colorectal cells that were incubated for 48 or 72 h either in normal conditions and µG simulated conditions. The comparative proteomic method based on the 18O labeling technique was applied to investigate the up-regulated proteins and down-regulated proteins in SH-SY5Y under simulated microgravity.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Colon Epithelial Cell

DISEASE(S): Colon Cancer

SUBMITTER: Riccardo Zenezini Chiozzi  

LAB HEAD: Aldo Laganà

PROVIDER: PXD005823 | Pride | 2017-07-26

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
2016-03-24_Caco2_TEST_fr1.raw Raw
2016-03-24_Caco2_TEST_fr10.raw Raw
2016-03-24_Caco2_TEST_fr11.raw Raw
2016-03-24_Caco2_TEST_fr12.raw Raw
2016-03-24_Caco2_TEST_fr2.raw Raw
Items per page:
1 - 5 of 159
altmetric image

Publications

Proteomic analysis and bioluminescent reporter gene assays to investigate effects of simulated microgravity on Caco-2 cells.

La Barbera Giorgia G   Capriotti Anna Laura AL   Michelini Elisa E   Piovesana Susy S   Calabretta Maria Maddalena MM   Zenezini Chiozzi Riccardo R   Roda Aldo A   Laganà Aldo A  

Proteomics 20170801 15-16

Microgravity is one of the most important features in spaceflight. Previous evidence from in-vitro studies has shown that significant changes occur under simulated microgravity. For this reason, human colon adenocarcinoma Caco-2 cells were selected as cell model of intestinal epithelial barrier and their response to altered gravity conditions was investigated, especially on the protein level. In this study, we combined label-free shotgun proteomics and bioluminescent reporter gene assays to iden  ...[more]

Similar Datasets

Transcriptomics Transcriptomic response of bioengineered human cartilage to parabolic flight microgravity is sex-dependent  
2022-09-30 | GSE206008 | GEO
Proteomics,Multiomics The influence of simulated microgravity on the proteome of Daphnia magna
2015-09-08 | PXD002096 | Pride
Transcriptomics Impact of modeled microgravity stress on innate immunity in a beneficial animal-microbe symbiosis
2024-02-14 | GSE247559 | GEO
Transcriptomics action of microgravity on root development-Action of microgravity on root development
2012-08-01 | GSE39631 | GEO
Transcriptomics action of microgravity on root development-Action of microgravity on root development
2012-07-31 | E-GEOD-39631 | biostudies-arrayexpress
Transcriptomics Human Peripheral Blood Lymphcoyte(PBL): Simulated Microgravity vs. Control
2014-04-10 | E-GEOD-56647 | biostudies-arrayexpress
Transcriptomics RNA sequencing of zebrafish embryos under simulated microgravity conditions
2020-12-22 | GSE145967 | GEO
Transcriptomics Synergistic effects of microgravity and space radiation (Nimblegen)
2017-03-01 | GSE90786 | GEO
Genomics Serum metabolomics associating with circulating microRNA profiles reveal the role of miR-383-5p in rat hippocampus under simulated microgravity
2020-07-08 | GSE153681 | GEO
Transcriptomics Effects of Simulated Microgravity on Colorectal Cancer Organoids Growth and Drug Response
2024-09-11 | GSE271642 | GEO