Dataset Information

The comparison of mitochondrial proteomics of fungal ETC Complex I mutant

ABSTRACT: Candidiasis is the most common invasive fungal infection in clinical sets. The causative agent C. albicans has a complex metabolism for energy production in which mitochondria play important roles for its virulence to the host. Three fungal- specific mitochondrial ETC complex CI subunits - Goa1p, Nuo1p (Nuxm) and Nuo2p (NUZM) have been associated with assembly of CI and mitochondrial function in this organism. They also carry on a number of cellular bioprocess including cell wall mannan. To better understand the biological functions of each subunit, we profile the proteomics of three gene knockout mutants, by comparison with one from wild type (SN250). We hypothesize the common and CI subunit- specific cellular activities which are bound to mitochondrial CI in this organism.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Caenorhabditis Elegans

SUBMITTER: jing wang

LAB HEAD: xiaodongshe

PROVIDER: PXD005842 | Pride | 2017-08-16

REPOSITORIES: pride

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A mitochondrial proteomics view of complex I deficiency in Candida albicans.

She Xiaodong X Zhang Pengyi P Gao Ying Y Zhang Lulu L Wang Qiong Q Chen Hui H Calderone Richard R Liu Weida W Li Dongmei D

Mitochondrion 20170809

Proteomic analyses were carried out on isolated mitochondrial samples of C. albicans from gene-deleted mutants (nuo1Δ, nuo2Δ and goa1Δ) as well as the parental strain in order to better understand the contribution of these three fungal-specific mitochondrial ETC complex I (CI) subunits to cellular activities. Herein, we identify 2333 putative proteins from four strains, in which a total of 663 proteins (28.5%) are putatively located in mitochondria. Comparison of protein abundances between mutan ...[more]

PMID: 28801230

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			Action	DRS
	MaxQuant_Output.zip	Other
	SN2501.raw	Raw
	SN2502.raw	Raw
	SN2503.raw	Raw
	goA311.raw	Raw