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Identification of NTAL protein interactors

ABSTRACT: The adaptor protein NTAL (non-T cell activation linker) is expresses in acute myeloid leukemia blasts, and its downregulation by alkylphospholipid treatment or protein expression knockdown reduces AKT activation. This new data elucidates new NTAL protein interactions.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture, Leukocyte

DISEASE(S): Acute Leukemia

SUBMITTER: Vitor Faca

LAB HEAD: Vitor Marcel Faça

PROVIDER: PXD005850 | Pride | 2021-09-10

REPOSITORIES: Pride

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			Action	DRS
	4676_U937_IgG_1.raw	Raw
	4677_U937_IgG_2.raw	Raw
	4678_U937_IgG_3.raw	Raw
	4680_NB4_IgG_1.raw	Raw
	4681_NB4_IgG_2.raw	Raw

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1 - 5 of 17

Publications

The Expression of NTAL and Its Protein Interactors Is Associated With Clinical Outcomes in Acute Myeloid Leukemia.

Thomé Carolina Hassibe CH Ferreira Germano Aguiar GA Pereira-Martins Diego Antonio DA Augusto Dos Santos Guilherme G Almeida-Silveira Douglas R DR Weinhäuser Isabel I Antônio de Souza Gustavo G Houtsma Roos R Schuringa Jan Jacob JJ Rego Eduardo M EM Faça Vitor M VM

Molecular & cellular proteomics : MCP 20210507

Non-T cell activation linker (NTAL) membrane protein depletion from lipid rafts by alkylphospholipids or downregulation by shRNA knockdown decreases cell viability through regulation of the Akt/PI3K pathway in mantle cell lymphoma and acute promyelocytic leukemia cells. Here, we confirmed that the knockdown of NTAL in acute myeloid leukemia (AML) cell lines was associated with decreased cell proliferation and survival. Similarly, a xenograft model using AML cells transduced with NTAL-shRNA and t ...[more]

PMID: 33971369

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2019-11-11 | PXD013245 | Pride

Human Serum, Following Absorption of Fish Cartilage Hydrolysate, Promotes Dermal Fibroblast Healing through Anti-Inflammatory and Immunomodulatory Proteins

Project description:Background/Objectives: Marine collagen peptides (MCPs) and glycosaminoglycans (GAGs) have been described as potential wound-healing (WH) agents. Fish cartilage hydrolysate (FCH) is a natural active food ingredient obtained from enzymatic hydrolysis which combines MCPs and GAGs. Recently, the clinical benefits of FCH supplementation for the skin, as well as its mode of action, have been demonstrated. Some of the highlighted mechanisms are common to the WH process. The aim of the study is therefore to investigate the influence of FCH supplementation on the skin healing processes and the underlying mechanisms. Methods: To this end, an ex vivo clinical approach, which takes into account the clinical digestive course of nutrients, coupled with primary cell culture on human dermal fibroblasts (HDFs) and ultra-deep proteomic analysis, was performed. The effects of human serum enriched in circulating metabolites resulting from FCH ingestion (FCH-enriched serum) were assessed on HDF WH via an in vitro scratch wound assay and on the HDF proteome via diaPASEF (Data Independent Acquisition—Parallel Accumulation Serial Fragmentation) proteomic analysis. Results: Results showed that FCH-enriched human serum accelerated wound closure. In support, proteins with anti-inflammatory and immunomodulatory properties and proteins prone to promote hydration and ECM stability showed increased expression in HDFs after exposure to FCH-enriched serum. Conclusions: Taken together, these data provide valuable new insights into the mechanisms that may contribute to FCH’s beneficial impact on human skin functionality by supporting WH. Further studies are needed to reinforce these preliminary data and investigate the anti-inflammatory and immunomodulatory properties of FCH.

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The Proteome and Transcriptome of Stress Granules and P-Bodies during Human T Lymphocytes Activation

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